Kojima A, Egashira Y
Immunology. 1979 Jul;37(3):569-76.
Delayed footpad reaction (FPR) to lysozyme (Lys) in mice was induced without antibody responses by lipid-conjugated lysozyme (D.Lys). This FPR was suppressed by priming s.c. with a high dose (10 mg) of Lys 2 weeks previously (unresponsiveness). Spleen cells from the unresponsive mice suppressed antigen-specifically FPR in mice previously immunized with D.Lys, and also suppressed passive transfer of FPR by D.Lys-immune lymphoid cells into normal mice. The suppressive activity of the spleen cells was abolished by treatment with anti-phi anti-serum and complement. The suppressor cells occurred also in the thymus of unresponsive mice. Unresponsiveness was induced in mice immediately after priming with Lys and persisted at least up to 7 weeks after the induction. In contrast, suppressor cells appeared only 2 weeks after induction of unresponsiveness in both the spleen and the thymus but were no longer detectable 3-7 weeks later, although donor mice remained fully unresponsive. These results suggest that antigen-specific suppressor T cells are involved in the regulation of the expression of FPR only for a definite period of time in unresponsive mice.
脂质偶联溶菌酶（D.Lys）可诱导小鼠对溶菌酶（Lys）产生延迟性足垫反应（FPR），且无抗体反应。两周前经皮下注射高剂量（10 mg）Lys进行预致敏（无反应性）可抑制这种FPR。无反应性小鼠的脾细胞可特异性抑制先前用D.Lys免疫的小鼠的FPR，也可抑制D.Lys免疫淋巴细胞向正常小鼠的FPR被动转移。脾细胞的抑制活性经抗ϕ抗血清和补体处理后被消除。无反应性小鼠的胸腺中也存在抑制细胞。小鼠在用Lys预致敏后立即诱导出无反应性，并至少持续至诱导后7周。相比之下，抑制细胞在无反应性诱导后仅在脾和胸腺中2周出现，但在3 - 7周后不再可检测到，尽管供体小鼠仍完全无反应。这些结果表明，抗原特异性抑制性T细胞仅在无反应性小鼠的特定时间段内参与FPR表达的调节。
