Universidade Federal do Maranhão (UFMA), Departamento de Biologia, São Luís, MA, Brazil.
Universidade Federal do Maranhão (UFMA), Departamento de Saúde Pública, São Luís, MA, Brazil.
J Pediatr (Rio J). 2020 Sep-Oct;96(5):630-637. doi: 10.1016/j.jped.2019.05.006. Epub 2019 Jul 30.
To investigate the association between the FTO gene polymorphism with obesity in Brazilian adolescents from the Northeast region.
This was a case-control study with adolescents aged 18 to 19 years. The case group consisted of 378 obese individuals and the control group of 378 non-obese individuals. Obesity was measured by percentage of body fat using the air displacement plethysmography technique. The study variables included data on socioeconomics, demographics, lifestyle, physical activity, waist circumference, waist-to-height ratio, and body mass index. To identify the rs9939609 polymorphism of the FTO gene, blood samples were obtained for genomic DNA extraction by the real-time PCR (Polymerase Chain Reaction) technique. Categorical variables were compared between the groups by the chi-squared test. The normality of the anthropometric measurements body mass index, waist circumference, waist-to-height ratio, and percentage of body fat was evaluated by the Shapiro-Wilk test. Comparison of the anthropometric measurements, stratified by the polymorphism genotypes, was performed by the Kruskal-Wallis test. The Hardy-Weinberg equilibrium was calculated. The significance level was set at 5%.
The variables gender, age, and physical activity showed significant differences between the groups (p<0.001). The samples of obese and non-obese adolescents were in Hardy-Weinberg equilibrium (p=0.0515). There was no significant difference between the genotypic (p=0.719) and allelic frequencies (p=0.812) regarding the case and control groups. When comparing the anthropometric measurements according to the genotypes (AA, AT, and TT), no significant difference was observed for body mass index (p=0.337), waist circumference (p=0.3473), percentage of body fat (p=0.7096), and waist-to-height ratio (p=0.2584).
The excess adiposity of the study adolescents was not influenced by their genotype.
研究巴西东北部地区青少年 FTO 基因多态性与肥胖的相关性。
这是一项病例对照研究，纳入 18 至 19 岁的青少年。病例组由 378 名肥胖个体组成，对照组由 378 名非肥胖个体组成。使用空气置换体积描记术测量体脂百分比来确定肥胖。研究变量包括社会经济学、人口统计学、生活方式、身体活动、腰围、腰高比和体重指数等数据。为了鉴定 FTO 基因的 rs9939609 多态性，通过实时 PCR（聚合酶链反应）技术获得血液样本，以提取基因组 DNA。通过卡方检验比较组间的分类变量。通过 Shapiro-Wilk 检验评估体重指数、腰围、腰高比和体脂百分比等人体测量指标的正态性。通过 Kruskal-Wallis 检验对按多态性基因型分层的人体测量指标进行比较。计算 Hardy-Weinberg 平衡。显著性水平设为 5%。
性别、年龄和身体活动等变量在组间存在显著差异（p<0.001）。肥胖和非肥胖青少年的样本均处于 Hardy-Weinberg 平衡（p=0.0515）。病例组和对照组的基因型（AA、AT 和 TT）和等位基因频率（p=0.719）均无显著差异（p=0.812）。根据基因型（AA、AT 和 TT）比较人体测量指标时，体重指数（p=0.337）、腰围（p=0.3473）、体脂百分比（p=0.7096）和腰高比（p=0.2584）均无显著差异。
研究青少年的多余脂肪量不受其基因型影响。
