FTO 基因多态性与巴西东北部青少年肥胖的相关性。

Association between the FTO gene polymorphism and obesity in Brazilian adolescents from the Northeast region.

机构信息

Universidade Federal do Maranhão (UFMA), Departamento de Biologia, São Luís, MA, Brazil.

Universidade Federal do Maranhão (UFMA), Departamento de Saúde Pública, São Luís, MA, Brazil.

出版信息

J Pediatr (Rio J). 2020 Sep-Oct;96(5):630-637. doi: 10.1016/j.jped.2019.05.006. Epub 2019 Jul 30.

DOI:10.1016/j.jped.2019.05.006

PMID:31374206

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9432123/

Abstract

OBJECTIVE

To investigate the association between the FTO gene polymorphism with obesity in Brazilian adolescents from the Northeast region.

METHOD

This was a case-control study with adolescents aged 18 to 19 years. The case group consisted of 378 obese individuals and the control group of 378 non-obese individuals. Obesity was measured by percentage of body fat using the air displacement plethysmography technique. The study variables included data on socioeconomics, demographics, lifestyle, physical activity, waist circumference, waist-to-height ratio, and body mass index. To identify the rs9939609 polymorphism of the FTO gene, blood samples were obtained for genomic DNA extraction by the real-time PCR (Polymerase Chain Reaction) technique. Categorical variables were compared between the groups by the chi-squared test. The normality of the anthropometric measurements body mass index, waist circumference, waist-to-height ratio, and percentage of body fat was evaluated by the Shapiro-Wilk test. Comparison of the anthropometric measurements, stratified by the polymorphism genotypes, was performed by the Kruskal-Wallis test. The Hardy-Weinberg equilibrium was calculated. The significance level was set at 5%.

RESULTS

The variables gender, age, and physical activity showed significant differences between the groups (p<0.001). The samples of obese and non-obese adolescents were in Hardy-Weinberg equilibrium (p=0.0515). There was no significant difference between the genotypic (p=0.719) and allelic frequencies (p=0.812) regarding the case and control groups. When comparing the anthropometric measurements according to the genotypes (AA, AT, and TT), no significant difference was observed for body mass index (p=0.337), waist circumference (p=0.3473), percentage of body fat (p=0.7096), and waist-to-height ratio (p=0.2584).

CONCLUSION

The excess adiposity of the study adolescents was not influenced by their genotype.

摘要

目的

研究巴西东北部地区青少年 FTO 基因多态性与肥胖的相关性。

方法

这是一项病例对照研究，纳入 18 至 19 岁的青少年。病例组由 378 名肥胖个体组成，对照组由 378 名非肥胖个体组成。使用空气置换体积描记术测量体脂百分比来确定肥胖。研究变量包括社会经济学、人口统计学、生活方式、身体活动、腰围、腰高比和体重指数等数据。为了鉴定 FTO 基因的 rs9939609 多态性，通过实时 PCR（聚合酶链反应）技术获得血液样本，以提取基因组 DNA。通过卡方检验比较组间的分类变量。通过 Shapiro-Wilk 检验评估体重指数、腰围、腰高比和体脂百分比等人体测量指标的正态性。通过 Kruskal-Wallis 检验对按多态性基因型分层的人体测量指标进行比较。计算 Hardy-Weinberg 平衡。显著性水平设为 5%。

结果

性别、年龄和身体活动等变量在组间存在显著差异（p<0.001）。肥胖和非肥胖青少年的样本均处于 Hardy-Weinberg 平衡（p=0.0515）。病例组和对照组的基因型（AA、AT 和 TT）和等位基因频率（p=0.719）均无显著差异（p=0.812）。根据基因型（AA、AT 和 TT）比较人体测量指标时，体重指数（p=0.337）、腰围（p=0.3473）、体脂百分比（p=0.7096）和腰高比（p=0.2584）均无显著差异。