纤连蛋白结构域蛋白 5 促进山羊脂肪干细胞的增殖和分化。
Fibronectin type III domain-containing protein 5 promotes proliferation and differentiation of goat adipose-derived stem cells.
机构信息
State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia, China.
State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia, China.
出版信息
Res Vet Sci. 2019 Aug;125:351-359. doi: 10.1016/j.rvsc.2019.07.011. Epub 2019 Jul 11.
Fibronectin type III domain-containing protein 5 (FNDC5) plays an important role in white-to-brown adipose tissue conversion in mice. However, there is no report on the role of this protein in Arbas Cashmere goat adipose tissue. We investigated the effect of FNDC5 on the proliferation and differentiation of goat adipose-derived stem cells (gADSCs). We found that FNDC5 promotes the proliferation of gADSCs and increases the levels of p-ERK and p-p38, while it has no effect on the levels of ERK, p38, AKT and p-AKT. What's more, FNDC5 promotes the differentiation of gADSCs into lipid droplets. Overexpression of FNDC5 increased the protein levels of ASC1, UCP1, PPARγ and SREBP1. Additionally, mRNA levels of PPARγ, SREBP1, ACC, and FABP4 increased significantly. FNDC5 knockdown was associated with opposite effects. These results suggest that FNDC5 promotes the proliferation of gADSCs via MAPK signaling pathway and also induces the differentiation of these cells.
纤维连接蛋白 III 型结构域蛋白 5（FNDC5）在小鼠白色脂肪组织向棕色脂肪组织转化中发挥重要作用。然而，目前尚无关于该蛋白在阿尔巴斯白绒山羊脂肪组织中作用的报道。本研究旨在探讨 FNDC5 对山羊脂肪干细胞（gADSCs）增殖和分化的影响。结果发现，FNDC5 促进 gADSCs 的增殖，并增加 ERK 和 p38 的磷酸化水平，而对 ERK、p38、AKT 和 p-AKT 的水平没有影响。此外，FNDC5 促进 gADSCs 向脂滴分化。FNDC5 的过表达增加了 ASC1、UCP1、PPARγ 和 SREBP1 的蛋白水平。同时，PPARγ、SREBP1、ACC 和 FABP4 的 mRNA 水平也显著增加。FNDC5 敲低则产生相反的效果。这些结果表明，FNDC5 通过 MAPK 信号通路促进 gADSCs 的增殖，并诱导这些细胞的分化。
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