1Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Kurupelit, 55200 Atakum, Samsun, Turkey.
2Department of Biochemistry, Faculty of Veterinary Medicine, Kırıkkale University, Kırıkkale, Turkey.
Acta Vet Hung. 2020 May 8;68(1):37-48. doi: 10.1556/004.2020.00005. Print 2020 Mar.
Squamous cell carcinoma (SCC) is the most common malignant neoplasm of the skin in cats. Tumour angiogenesis is the pivotal event for tumour progression and metastasis. We assessed protein and gene expression of angiogenic growth factors including bFGF, VEGF-C, TGF-β, PDGF-A, PDGF-C and PDGFR-α that possibly contribute to the angiogenic phenotype of feline SCC (FSCC) and could, therefore, be a good target in the treatment of SCC. In the present study, a total of 27 FSCC cases were investigated. Tumour cases were histopathologically classified as well differentiated (10/27), moderately differentiated (5/27), and poorly differentiated (12/27). The expression levels of the growth factors were detected using immunohistochemistry and assessed semi-quantitatively. Growth factor expression levels were evaluated at different locations: in the oral region, in areas exposed to solar UV radiation including the ears, eyelids and nasal planum, and other miscellaneous locations. Our findings have revealed that FSCC arising from different anatomical sites of the body and showing differences in aggressiveness, metastasis, and prognosis may be angiogenesis dependent, and angiogenic key regulators could play a role in the development of FSCC.
鳞状细胞癌 (SCC) 是猫皮肤最常见的恶性肿瘤。肿瘤血管生成是肿瘤进展和转移的关键事件。我们评估了血管生成生长因子的蛋白和基因表达，包括 bFGF、VEGF-C、TGF-β、PDGF-A、PDGF-C 和 PDGFR-α，这些因子可能有助于猫 SCC (FSCC) 的血管生成表型，因此可能成为 SCC 治疗的一个良好靶点。在本研究中，共研究了 27 例 FSCC 病例。肿瘤病例根据组织病理学分为分化良好 (10/27)、中度分化 (5/27) 和低分化 (12/27)。使用免疫组织化学检测生长因子的表达水平，并进行半定量评估。在不同部位评估生长因子的表达水平：口腔部位、暴露于太阳紫外线辐射的部位，包括耳朵、眼睑和鼻平面，以及其他杂项部位。我们的研究结果表明，来自身体不同解剖部位的 FSCC 具有不同的侵袭性、转移和预后，可能依赖于血管生成，血管生成的关键调节剂可能在 FSCC 的发展中发挥作用。
