使用血浆下一代测序技术进行感染的非侵入性诊断：单中心经验
Noninvasive Diagnosis of Infection Using Plasma Next-Generation Sequencing: A Single-Center Experience.
作者信息
Rossoff Jenna, Chaudhury Sonali, Soneji Maulin, Patel Sameer J, Kwon Soyang, Armstrong Amy, Muller William J
机构信息
Division of Hematology, Oncology and Transplantation, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois.
Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
出版信息
Open Forum Infect Dis. 2019 Aug 1;6(8). doi: 10.1093/ofid/ofz327.
Abstract
Pediatric patients frequently present with illnesses strongly suggesting infection, but without a clearly identified etiology. Our center has recently added a commercially available plasma metagenomic sequencing assay to its available diagnostic testing. Our experience with the first 100 tests suggests that this technology has good clinical performance with >90% sensitivity.
摘要
儿科患者经常出现强烈提示感染但病因未明确的疾病。我们中心最近在其现有的诊断检测项目中增加了一项商用血浆宏基因组测序检测。我们对最初100次检测的经验表明，这项技术具有良好的临床性能，灵敏度超过90%。
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本文引用的文献
1
Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease.微生物无细胞 DNA 测序检测用于感染性疾病的分析和临床验证。
Nat Microbiol. 2019 Apr;4(4):663-674. doi: 10.1038/s41564-018-0349-6. Epub 2019 Feb 11.
2
Microbiological Diagnostic Performance of Metagenomic Next-generation Sequencing When Applied to Clinical Practice.宏基因组下一代测序在临床应用中的微生物诊断性能。
Clin Infect Dis. 2018 Nov 13;67(suppl_2):S231-S240. doi: 10.1093/cid/ciy693.
3
Application of next generation sequencing in clinical microbiology and infection prevention.下一代测序技术在临床微生物学与感染预防中的应用。
J Biotechnol. 2017 Feb 10;243:16-24. doi: 10.1016/j.jbiotec.2016.12.022. Epub 2016 Dec 29.
4
Towards precision medicine.迈向精准医学。
Nat Rev Genet. 2016 Aug 16;17(9):507-22. doi: 10.1038/nrg.2016.86.
5
Noninvasive monitoring of infection and rejection after lung transplantation.肺移植术后感染与排斥反应的无创监测
Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):13336-41. doi: 10.1073/pnas.1517494112. Epub 2015 Oct 12.
6
Emerging technologies for the clinical microbiology laboratory.临床微生物实验室的新兴技术。
Clin Microbiol Rev. 2014 Oct;27(4):783-822. doi: 10.1128/CMR.00003-14.
7
The next-generation sequencing revolution and its impact on genomics.下一代测序革命及其对基因组学的影响。
Cell. 2013 Sep 26;155(1):27-38. doi: 10.1016/j.cell.2013.09.006.
8
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.流感嗜血杆菌Rd的全基因组随机测序与组装
Science. 1995 Jul 28;269(5223):496-512. doi: 10.1126/science.7542800.
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