Fleischmann R D, Adams M D, White O, Clayton R A, Kirkness E F, Kerlavage A R, Bult C J, Tomb J F, Dougherty B A, Merrick J M
Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Science. 1995 Jul 28;269(5223):496-512. doi: 10.1126/science.7542800.
An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.
一种基于对来自整条染色体的未选择DNA片段进行测序和组装的基因组分析方法，已被用于获得流感嗜血杆菌Rd菌株基因组的完整核苷酸序列（1,830,137个碱基对）。这种方法无需进行初始的定位工作，因此适用于大量尚无基因组图谱的微生物物种。流感嗜血杆菌Rd菌株的基因组序列（基因组序列数据库登录号L42023）代表了来自自由生活生物体的唯一完整基因组序列。
