Liu Yahui, Ge Mengying, Zhang Tingting, Chen Lu, Xing Yuping, Liu Liu, Li Fengliang, Cheng Luogen
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China.
Genomics. 2020 Mar;112(2):1300-1308. doi: 10.1016/j.ygeno.2019.07.019. Epub 2019 Jul 31.
Deltamethrin (DM) is widely used in a variety of pest control, resulting in serious drug resistance. Keap1-Nrf2-ARE is the antioxidant stress pathway. We identified 268 genes differentially expressed (DEGs) in Drosophila Kc cells treated with DM, including up-regulated 180 genes and down-regulated 88 genes compared with the control group (fold-change≥2, qValue≤0.001) by RNA-seq, they are mainly linked to metabolic process, stimulation response, immune system process. When the cells are treated with DM in the case of overexpression of the Keap1 gene, the cytochrome P450 family genes were significantly down-regulated, and some diseases-related genes and non-coding genes also changed. Our data shown that Keap1-Nrf2-ARE pathway may play an important role in DM stress, which will provide a new direction for studying the mechanism of insect resistance.
溴氰菊酯（DM）被广泛用于各种害虫防治，导致严重的耐药性。Keap1-Nrf2-ARE是抗氧化应激途径。我们通过RNA测序鉴定了在经DM处理的果蝇Kc细胞中差异表达的268个基因（DEG），与对照组相比，包括180个上调基因和88个下调基因（倍数变化≥2，q值≤0.001），它们主要与代谢过程、刺激反应、免疫系统过程相关。当在Keap1基因过表达的情况下用DM处理细胞时，细胞色素P450家族基因显著下调，一些疾病相关基因和非编码基因也发生了变化。我们的数据表明，Keap1-Nrf2-ARE途径可能在DM应激中起重要作用，这将为研究昆虫抗性机制提供新的方向。
