College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
Bioresour Technol. 2019 Nov;291:121886. doi: 10.1016/j.biortech.2019.121886. Epub 2019 Jul 25.
The objective of this study was to identify the effect of clay additive to explore the nitrogen cycling related ammonification, denitrifying genes and their relation NO and ammonia emission during the chicken manure (CM) composting. The consequences indicated that the additive clay could optimize the environment and improve the NH-N into NO-N transformation. Meanwhile, the results observed that 8% clay amendment was significant correlated the abundance of nifH and nosZ genes. Compared with the other treatments, the 6% and 10% clay treatments observed low abundance of nifH. The heat map indicated that clay amendment could enhance the function genes abundance. The redundancy analysis showed that NO and ammonia emission among all physiochemical parameters had great relationship with functional genes. Therefore, the addition of 10% clay into CM composting system could efficiently enhance the abundance of nirK and narG genes, and improve the composting with its application.
本研究的目的是确定粘土添加剂对鸡粪（CM）堆肥过程中氮循环相关氨化、反硝化基因及其与 NO 和氨排放关系的影响。结果表明，添加剂粘土可以优化环境，促进 NH-N 向 NO-N 的转化。同时，结果观察到 8%的粘土添加量与 nifH 和 nosZ 基因的丰度呈显著正相关。与其他处理相比，6%和 10%的粘土处理观察到 nifH 的丰度较低。热图表明，粘土添加可以增强功能基因的丰度。冗余分析表明，NO 和氨排放与所有理化参数之间具有很大的关系。因此，将 10%的粘土添加到 CM 堆肥系统中可以有效地提高 nirK 和 narG 基因的丰度，并提高其应用的堆肥效果。
