College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, PR China.
College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, PR China.
Bioresour Technol. 2022 Oct;361:127678. doi: 10.1016/j.biortech.2022.127678. Epub 2022 Jul 21.
The objective of this work was to explore how selection of feedstock affects nitrogen cycle genes during composting, which eventually determines the nitrogen loss. Four composting mixes (CM: chicken manure; SM: sheep manure; MM: mixed manure with CM: SM = 1:3 w/w, MM: CM: SM = 3:1 w/w) were investigated. Results showed that adding 25 % and 75 % SM to CM reduced 26.5 % and 57.9 % nitrogen loss, respectively. CM contained more ammonification genes and nrfA gene, while SM had more denitrification genes. Nitrogen fixation genes in CM were slightly higher than that in SM at the initial stage, but they sharply dropped off as the composting entered the high temperature stage. MM showed significantly reduced ammonification genes than CM, and increased nitrogen fixation and NH assimilation genes. Therefore, adding SM to CM could change the abundance of genes and enzymes related to nitrogen cycle to reduce nitrogen loss.
本研究旨在探讨在堆肥过程中，原料选择如何影响氮循环基因，从而最终决定氮素损失。本研究采用四种堆肥混合物（CM：鸡粪；SM：羊粪；MM：CM 和 SM 按 1:3（w/w）混合；MM：CM 和 SM 按 3:1（w/w）混合）进行研究。结果表明，添加 25%和 75%的 SM 可分别降低 26.5%和 57.9%的氮素损失。CM 中含有更多的氨化基因和 nrfA 基因，而 SM 中则含有更多的反硝化基因。CM 中的固氮基因在初始阶段略高于 SM，但随着堆肥进入高温阶段，固氮基因急剧下降。MM 中的氨化基因明显低于 CM，而固氮和 NH 同化基因则增加。因此，添加 SM 可以改变与氮循环相关的基因和酶的丰度，从而减少氮素损失。
