School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, PR China.
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
J Colloid Interface Sci. 2019 Nov 1;555:82-93. doi: 10.1016/j.jcis.2019.07.061. Epub 2019 Jul 24.
This study introduced multistage pH-responsive nanohybrids (MSN-hyd-MOP) based on mesoporous silica nanoparticles (MSNs) modified with polymers with charge-reversal property via an acid-labile hydrazone linker, which were applied as a drug delivery system loaded anticancer drugs. In this study, MSN-hyd-MOP nanohybrids were completely investigated for their synthesis, pH response, drug release behavior, cytotoxicity capability and endocytic behavior. Responding to the acidic extracellular microenvironment of solid tumor (pH 6.5), MSN-hyd-MOP nanohybrids exhibited surface charge-reversal characteristic from negative (-10.2 mV, pH 7.4) to positive (16.6 mV, pH 6.5). The model drug doxorubicin (Dox) was efficiently loaded within the channels of MSN-hyd-MOP (encapsulation efficiency about 87%). The increased acidity in endo-/lysosome promote Dox-loaded MSN-hyd-MOP (MSN-hyd-MOP@Dox) release Dox quickly. In vitro study revealed the drug delivery system had good biocompatibility and could deliver the payload to tumor cells. Overall, the described nanohybrids can be used as a potential anticancer drug delivery system.
本研究通过酸不稳定腙键将具有荷负电反转性质的聚合物修饰到介孔硅纳米粒子（MSNs）上，制备了基于多阶段 pH 响应纳米杂化材料（MSN-hyd-MOP）。该纳米杂化材料作为负载抗癌药物的药物递送系统。在这项研究中，对 MSN-hyd-MOP 纳米杂化材料的合成、pH 响应、药物释放行为、细胞毒性和内吞作用进行了全面研究。MSN-hyd-MOP 纳米杂化材料在酸性条件下（pH 6.5）表现出表面电荷反转特性，从带负电（pH 7.4 时为-10.2 mV）转变为带正电（pH 6.5 时为 16.6 mV）。模型药物阿霉素（Dox）能够高效地负载在 MSN-hyd-MOP 的孔道内（包封效率约为 87%）。内体/溶酶体中酸度的增加促进了负载 Dox 的 MSN-hyd-MOP（MSN-hyd-MOP@Dox）快速释放 Dox。体外研究表明，该药物递送系统具有良好的生物相容性，并能够将药物有效递送到肿瘤细胞。总之，所描述的纳米杂化材料可用作潜在的抗癌药物递送系统。
