Posgrado en Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Campus Montecillo, Km 36.5 Carretera México-Texcoco, 56230, Montecillo, Estado de México, Mexico.
Posgrado en Fitosanidad-Fitopatología, Colegio de Postgraduados, Campus Montecillo, Km 36.5 Carretera México-Texcoco, 56230, Montecillo, Estado de México, Mexico.
Exp Appl Acarol. 2019 Sep;79(1):69-86. doi: 10.1007/s10493-019-00409-w. Epub 2019 Aug 3.
Citrus leprosis virus C (CiLV-C) is an economically important pathogen and the main causative agent of leprosis disease in citrus orchards. The main vector of this disease, the mite Brevipalpus yothersi, is widely distributed in Mexican orchards on a wide range of citrus species. Despite the importance of both the virus and the mite, field studies recording their occurrence and co-occurrence are practically non-existent. We systematically sampled orange orchards for both CiLV-C and B. yothersi throughout the year. The distribution of the CiLV-C and B. yothersi was evaluated on each sampling occasion and their spatiotemporal associations were determined. Specifically, 100-112 orange trees, distributed in 18 rows (five or six trees per row), were sampled monthly between March 2017 and February 2018 (11 sampling dates). Twenty leaves per tree were sampled on each occasion. The number of mites per tree and the percentage of leaves per tree with disease symptoms were recorded. On each sampling occasion, spatiotemporal associations between mites and disease were determined using the Spatial Analysis by Distance Indices (SADIE) method. CiLV-C and B. yothersi were identified using molecular methods. Throughout the study, the distribution of CiLV-C was aggregated and the distribution of B. yothersi was random. No association was found between the virus and the mite on any of the sampling dates. In total, 173 mites were collected, but only 43 mites were found to be carrying CiLV-C. The reason for this lack of association between the virus and the mite, as well as the impact of our findings on the epidemiology of the disease in orange orchards, are discussed.
克莱氏柠檬丛矮化病毒 C（CiLV-C）是一种重要的经济病原体，也是柑橘园中麻皮病的主要致病因子。该疾病的主要媒介——红蜘蛛 B. yothersi 广泛分布于墨西哥果园中，存在于多种柑橘物种上。尽管该病毒和螨虫都很重要，但实际上几乎没有关于它们发生和共同发生的田间研究记录。我们全年系统地对橙园中的 CiLV-C 和 B. yothersi 进行了采样。在每次采样时评估 CiLV-C 和 B. yothersi 的分布情况，并确定它们的时空关联。具体来说，在 2017 年 3 月至 2018 年 2 月（共 11 个采样日期）的 11 次采样中，我们在 18 行（每行 5-6 棵树）中随机选取了 100-112 棵橙树。每次采样时，每棵树取 20 片叶子。记录每棵树的螨虫数量和每棵树出现病害症状的叶片百分比。在每次采样时，使用距离指数的空间分析（SADIE）方法确定螨虫和病害之间的时空关联。使用分子方法鉴定 CiLV-C 和 B. yothersi。在整个研究过程中，CiLV-C 的分布是聚集的，而 B. yothersi 的分布是随机的。在任何采样日期都未发现病毒与螨虫之间存在关联。共采集到 173 只螨虫，但只有 43 只螨虫携带 CiLV-C。我们讨论了这种病毒与螨虫之间缺乏关联的原因，以及我们的发现对柑橘园中这种疾病的流行病学的影响。
