携带外源基因的衰减型 MuV-S79 载体。
Attenuated MuV-S79 as vector stably expressing foreign gene.
机构信息
Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310052, China.
出版信息
World J Pediatr. 2019 Oct;15(5):511-515. doi: 10.1007/s12519-019-00287-7. Epub 2019 Aug 3.
BACKGROUND
To describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes.
METHODS
Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity.
RESULTS
Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages.
CONCLUSION
rMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.
背景
描述腮腺炎病毒（MuV）作为载体表达增强型绿色荧光蛋白（EGFP）或红色荧光蛋白（RFP）基因。
方法
应用分子克隆技术构建重组腮腺炎病毒（rMuV）的 cDNA 克隆。基于我们的腮腺炎病毒 S79 反向遗传系统来拯救 rMuV。通过生长曲线、噬斑试验、荧光显微镜和荧光强度测定来确定 rMuV 的特性。
结果
三种重组病毒在 Vero 细胞中复制良好，与亲本 rMuV-S79 相似，高水平表达异源基因，并且在至少 15 代中遗传稳定。
结论
rMuV-S79 是一种有前途的平台，可以容纳标记基因、其他抗原和免疫调节剂等外源基因，用于解决各种疾病。
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