Heilongjiang Province Key Laboratory of Forest Protection, School of Forest, Northeast Forestry University, Hexing Road 26, Xiangfang District, Harbin 150040, PR China.
Heilongjiang Province Key Laboratory of Forest Protection, School of Forest, Northeast Forestry University, Hexing Road 26, Xiangfang District, Harbin 150040, PR China.
Pestic Biochem Physiol. 2019 Jul;158:25-31. doi: 10.1016/j.pestbp.2019.04.003. Epub 2019 Apr 10.
Sodium pheophorbide a (SPA) is a natural photosensitizer. To explore its antifungal activity and mechanism, we studied its inhibitory effects on spore germination and mycelial growth of Pestalotiopsis neglecta. We used sorbitol, 2-thiobarbituric acid (TBA) and electron microscopy to determine its effects on cell wall integrity, cell membrane lipid peroxidation and mycelial morphology. Finally, the effects of SPA on enzyme activity in mycelia were determined. The results showed that SPA effectively inhibited spore germination and mycelial growth of P. neglecta under light conditions (4000 lx, 24 h). Scanning electron microscopy (SEM) revealed that SPA treatment resulted in a roughened, twisted and knotted mycelial surface and abnormal mycelial growth. SPA influenced cell wall integrity, and the content of MDA, a cell membrane lipid peroxidation product was significantly increased (P < 0.05). SPA also significantly inhibited SOD, POD and PG activity, but enhanced PPO activity (P < 0.05). In conclusion, SPA may have potential to become a biological pesticide.
叶绿酸甲酯（SPA）是一种天然光敏剂。为了探索其抗真菌活性和机制，我们研究了其对串珠镰刀菌孢子萌发和菌丝生长的抑制作用。我们使用山梨糖醇、2-硫代巴比妥酸（TBA）和电子显微镜来确定其对细胞壁完整性、细胞膜脂质过氧化和菌丝形态的影响。最后，测定了 SPA 对菌丝酶活性的影响。结果表明，SPA 在光照条件下（4000 lx，24 h）能有效抑制串珠镰刀菌的孢子萌发和菌丝生长。扫描电子显微镜（SEM）显示 SPA 处理导致菌丝表面粗糙、扭曲和打结，菌丝生长异常。SPA 影响细胞壁完整性，细胞膜脂质过氧化产物 MDA 的含量显著增加（P < 0.05）。SPA 还显著抑制 SOD、POD 和 PG 活性，但增强 PPO 活性（P < 0.05）。综上所述，SPA 可能具有成为生物农药的潜力。
