Topal F, Goren H, Yucel F, Sahinturk V, Aydar Y
Bratisl Lek Listy. 2019;120(8):593-600. doi: 10.4149/BLL_2019_097.
The incidence of obesity and obesity-assosiated pathologies continues to increase with profound adverse effects on health status in the developed countries.
We aimed to investigate the effect of high fat diet on the adrenal gland morphology.
We fed the mice with either high-fat diet (60 % kcal from fat) or low-fat diet (10 % kcal from fat) for nine weeks. Unbiased stereological methods were used to evaluate the adrenal gland morphology. The sections were evaluated using Cavalieri's method and volume fraction approach. We calculated mean volume of adrenal gland, mean volume of adrenal medulla, VVadrenal medulla/adrenal gland, mean diameter of cromaffin cells, number of chromaffin cells in per unit volume (NVcc mm‒3), total number of cromaffin cells, VVzona glomerulosa/adrenal cortex, VVzona fasciculata/adrenal cortex , VVzona reticulosa/adrenal cortex.
The weight of adrenal gland, body weight intraperitoneal adipose tissue and adrenal gland weight in the obese mice significantly increased when compared with the control group. No changes were observed in the mean volume of adrenal gland, mean volume of adrenal medulla, VVzona glomerulosa/adrenal cortex, VVzona fasciculata/adrenal cortex, total number of cromaffin cells and diameter of cromaffin cells. However, NVcc mm-3 and VVzona reticulosa/adrenal cortex in the obese mice considerably increased compared with the control group.
The present results suggest that high fat diet adversely affects the adrenal gland morphology (Tab. 2, Fig. 6, Ref. 28).
在发达国家，肥胖及与肥胖相关的病症发病率持续上升，对健康状况产生了严重的不利影响。
我们旨在研究高脂饮食对肾上腺形态的影响。
我们用高脂饮食（脂肪提供60%千卡热量）或低脂饮食（脂肪提供10%千卡热量）喂养小鼠9周。采用无偏倚的体视学方法评估肾上腺形态。使用卡瓦列里方法和体积分数法对切片进行评估。我们计算了肾上腺的平均体积、肾上腺髓质的平均体积、肾上腺髓质/肾上腺的体积分数、嗜铬细胞的平均直径、每单位体积嗜铬细胞的数量（NVcc mm‒3）、嗜铬细胞总数、球状带/肾上腺皮质的体积分数、束状带/肾上腺皮质的体积分数、网状带/肾上腺皮质的体积分数。
与对照组相比，肥胖小鼠的肾上腺重量、体重、腹膜内脂肪组织和肾上腺重量显著增加。肾上腺的平均体积、肾上腺髓质的平均体积、球状带/肾上腺皮质的体积分数、束状带/肾上腺皮质的体积分数、嗜铬细胞总数和嗜铬细胞直径未观察到变化。然而，与对照组相比，肥胖小鼠的NVcc mm-3和网状带/肾上腺皮质的体积分数显著增加。
目前的结果表明高脂饮食对肾上腺形态有不利影响（表2，图6，参考文献28）。
