Fujii Yasuyuki, Suzuki Kenta, Adachi Takahiro, Taira Shu, Osakabe Naomi
Department of Bioscience and Engineering, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama 337-8570, Japan.
Department of Immunology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
J Clin Biochem Nutr. 2019 Jul;65(1):29-33. doi: 10.3164/jcbn.19-19. Epub 2019 Jun 7.
Cinnamtannin A2, an (-)-epicatechin tetramer, was reported to have potent physiological activity. Cinnamtannin A2 is rarely absorbed from the gastrointestinal tract into the blood and the mechanisms of its beneficial activities are unknown. Cinnamtannin A2 reported to increase sympathetic nervous activity, which was induced by various stressors. In present study, we examined the stress response in the mouse paraventricular nucleus following a single oral dose of cinnamtannin A2 by monitoring mRNA expression of corticotropin-releasing hormone (CRH) and c-fos using hybridization. Corticotropin-releasing hormone mRNA showed a tendency to increase at 15 min and significantly increased at 60 min following a single oral administration of 100 µg/kg cinnamtannin A2. After a single dose of 10 µg/kg cinnamtannin A2, there was significant upregulation of CRH mRNA at 60 min. These results suggested that cinnamtannin A2 was recognized as a stressor in central nervous system and this may lead to its beneficial effects on circulation and metabolism.
肉桂单宁A2是一种（-）-表儿茶素四聚体，据报道具有强大的生理活性。肉桂单宁A2很少从胃肠道吸收进入血液，其有益活性的机制尚不清楚。据报道，肉桂单宁A2会增加由各种应激源诱导的交感神经活动。在本研究中，我们通过使用杂交技术监测促肾上腺皮质激素释放激素（CRH）和c-fos的mRNA表达，研究了单次口服肉桂单宁A2后小鼠室旁核中的应激反应。单次口服100μg/kg肉桂单宁A2后，促肾上腺皮质激素释放激素mRNA在15分钟时呈增加趋势，在60分钟时显著增加。单次给予10μg/kg肉桂单宁A2后，60分钟时CRH mRNA有显著上调。这些结果表明，肉桂单宁A2在中枢神经系统中被识别为一种应激源，这可能导致其对循环和代谢产生有益影响。
