Wang Jigong, La Jun-Ho, Hamill Owen P
Department of Neuroscience, Cell Biology and Anatomy, The University of Texas Medical Branch, Galveston, TX, United States.
Front Mol Neurosci. 2019 Jul 19;12:178. doi: 10.3389/fnmol.2019.00178. eCollection 2019.
Using a high resolution hybridization technique we have measured , , and transcripts in mouse dorsal root ganglion (DRG) neurons. Consistent with previous studies, transcripts were highly expressed in DRG neurons of all sizes, including most notably the largest diameter neurons implicated in mediating touch and proprioception. In contrast, transcripts were selectively expressed in smaller DRG neurons, which are implicated in mediating nociception. Moreover, the small neurons expressing were mostly distinct from those neurons that strongly expressed , one of the channels implicated in heat-nociception. Interestingly, while and expressing neurons form essentially non-overlapping populations, showed co-expression in both populations. Using an functional test for the selective expression, we found that Yoda1, a PIEZO1-specific agonist, induced a mechanical hyperalgesia that displayed a significantly prolonged time course compared with that induced by capsaicin, a TRPV1-specific agonist. Taken together, our results indicate that PIEZO1 should be considered a potential candidate in forming the long sought channel mediating mechano-nociception.
我们使用高分辨率杂交技术检测了小鼠背根神经节（DRG）神经元中的、和转录本。与之前的研究一致，转录本在各种大小的DRG神经元中均高表达，其中最显著的是参与介导触觉和本体感觉的最大直径神经元。相比之下，转录本在较小的DRG神经元中选择性表达，这些神经元参与介导伤害感受。此外，表达的小神经元大多与强烈表达的神经元不同，是参与热伤害感受的通道之一。有趣的是，虽然表达和的神经元形成基本不重叠的群体，但在这两个群体中都有共表达。通过对选择性表达进行功能测试，我们发现PIEZO1特异性激动剂Yoda1诱导的机械性痛觉过敏与TRPV1特异性激动剂辣椒素诱导的相比，其时间进程显著延长。综上所述，我们的结果表明，PIEZO1应被视为形成长期寻找的介导机械性伤害感受通道的潜在候选者。
