Hartley M Gill, Ralph Esther, Norville Isobel H, Prior Joann L, Atkins Timothy P
CBR, Defence Science and Technology Laboratory, Salisbury, United Kingdom.
College of Life and Environmental Sciences, University of Exeter, Exeter, United Kingdom.
Front Microbiol. 2019 Jul 16;10:1552. doi: 10.3389/fmicb.2019.01552. eCollection 2019.
Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of DNA in the host. However, the colonization and dissemination of other bacterial infections in animal models are tracked using viable counts, enabling an accurate assessment of viable bacterial load within tissues. Following recent advances in the culture methods, it has become possible to do the same with . Here we compare and contrast the different information gained by using PCR or viable counts to study this disease. Viable bacteria were cleared from organs much faster than previously reported when assessed by bacterial DNA, but weight loss and clinical signs improved while animals were still heavily infected.
从历史上看，Q热动物模型中的疾病进展一直是通过聚合酶链反应（PCR）来监测宿主体内DNA的存在情况。然而，动物模型中其他细菌感染的定植和传播是通过活菌计数来追踪的，从而能够准确评估组织内活菌载量。随着培养方法的最新进展，对[此处原文缺失相关内容]也可以进行同样的操作。在这里，我们比较和对比了使用PCR或活菌计数来研究这种疾病所获得的不同信息。当通过细菌DNA评估时，器官中的活菌清除速度比之前报道的要快得多，但在动物仍受到严重感染时，体重减轻和临床症状有所改善。
