Stukalov S V
Biull Eksp Biol Med. 1988 Aug;106(8):220-1.
2.4 and 6 mg/kg thiophosphamide (T) was administered intravenously to New Zealand rabbits. A decrease in sister chromatid exchange (SCE) and chromosome aberration (CA) rate began immediately after the mutagenic action of T was over. The expected SCE rate was more than the investigated one. The difference between expected and investigated SCE rate increased with the dose of T. A calculation of SCE was based on the amount of the administered T, the rate of T removal and cell sensitivity to T. The death of cells with high number of SCE resulted in a fast decrease in SCE rate in the first 4 days. Reparative processes and cell proliferation in lymphocyte tissue resulted in a slow decrease in SCE rate after the 4th day. A number of nuclear cells in the blood was the smallest on the 4 th day, at the same time relative increase in CA rate was observed. The time of sampling and the dose of the substance tested should be taken into account for a more accurate estimation of mutagenic activity of some chemicals in in vivo cytogenetic tests.
将2.4毫克/千克和6毫克/千克的硫代磷酰胺（T）静脉注射给新西兰兔。在T的诱变作用结束后，姐妹染色单体交换（SCE）率和染色体畸变（CA）率立即开始下降。预期的SCE率高于所研究的SCE率。预期SCE率与所研究SCE率之间的差异随T的剂量增加而增大。SCE的计算基于所给予T的量、T的清除率以及细胞对T的敏感性。具有高SCE数目的细胞死亡导致前4天SCE率快速下降。淋巴细胞组织中的修复过程和细胞增殖导致第4天后SCE率缓慢下降。第4天血液中的核细胞数量最少，同时观察到CA率相对增加。在体内细胞遗传学试验中，为了更准确地评估某些化学物质的诱变活性，应考虑采样时间和受试物质的剂量。
