Liu Wei, Sun Baozheng, Yang Manman, Zhang Ziyue, Zhang Xiuxiu, Pang Tingsong, Wang Shengzheng
Faculty of Pharmacy, Shaanxi University of Science and Technology, Xi'an 710021, China.
Department of Medicinal Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
Evid Based Complement Alternat Med. 2019 Jul 15;2019:5320203. doi: 10.1155/2019/5320203. eCollection 2019.
This study aimed to investigate the antifungal activity of hydroalcoholic extract from (SJA) against different fungi. The minimum inhibitory concentration (MIC) for SJA was determined by the broth microdilution method. The antifungal effects of SJA against were further confirmed by cell growth test and time-kill curve test. The effects of SJA on the fungal morphology and ultrastructure were also evaluated. SJA has a broad-spectrum antifungal activity. The MICs of SJA against different fungi, including fluconazole-sensitive and -resistant , other species, and , ranged from 208 g/ml to 1665 g/ml. Furthermore, SJA displayed fungicidal activity against varied fungi and obviously inhibited the hyphal growth of fungi. The mechanism study revealed that the antifungal activity of SJA might be associated with its effect on the cell morphology and ultrastructure.
本研究旨在探讨[植物名称未给出，推测为某种植物，暂用(SJA)表示]水醇提取物对不同真菌的抗真菌活性。采用肉汤微量稀释法测定SJA的最低抑菌浓度(MIC)。通过细胞生长试验和时间-杀菌曲线试验进一步证实了SJA对[相关真菌未给出，推测为某些特定真菌，暂用“ ”表示]的抗真菌作用。还评估了SJA对真菌形态和超微结构的影响。SJA具有广谱抗真菌活性。SJA对不同真菌的MIC，包括对氟康唑敏感和耐药的[相关真菌未给出，推测为某些特定真菌，暂用“ ”表示]、其他[相关真菌未给出，推测为某些特定真菌，暂用“ ”表示]种以及[相关真菌未给出，推测为某些特定真菌，暂用“ ”表示]，范围为208μg/ml至1665μg/ml。此外，SJA对多种真菌表现出杀菌活性，并明显抑制真菌的菌丝生长。机制研究表明，SJA的抗真菌活性可能与其对细胞形态和超微结构的影响有关。
