Fortunato Gabriele Maria, Da Ros Francesco, Bisconti Samuele, De Acutis Aurora, Biagini Francesco, Lapomarda Anna, Magliaro Chiara, De Maria Carmelo, Montemurro Francesca, Bizzotto Dario, Braghetta Paola, Vozzi Giovanni
Research Centre 'E. Piaggio', Department of Ingegneria dell'Informazione of University of Pisa, Pisa, Italy.
Department of Molecular Medicine, University of Padova, Padova, Italy.
Front Bioeng Biotechnol. 2019 Jul 17;7:174. doi: 10.3389/fbioe.2019.00174. eCollection 2019.
The aim of this study is the analysis and characterization of a hydrolyzed keratin-based biomaterial and its processing using electrospinning technology to develop tissue models. This biomaterial, extracted from poultry feathers, was mixed with type A porcine gelatin and cross-linked with γ-glycidyloxy-propyl-trimethoxy-silane (GPTMS) to be casted initially in the form of film and characterized in terms of swelling, contact angle, mechanical properties, and surface charge density. After these chemical-physical characterizations, electrospun nanofibers structures were manufactured and their mechanical properties were evaluated. Finally, cell response was analyzed by testing the efficacy of keratin-based structures in sustaining cell vitality and proliferation over 4 days of human epithelial, rat neuronal and human primary skin fibroblast cells.
本研究的目的是分析和表征一种基于水解角蛋白的生物材料，并利用静电纺丝技术对其进行加工以开发组织模型。这种从家禽羽毛中提取的生物材料与A型猪明胶混合，并用γ-缩水甘油氧基丙基三甲氧基硅烷（GPTMS）交联，最初制成薄膜形式，并对其溶胀、接触角、机械性能和表面电荷密度进行表征。在进行这些化学物理表征之后，制造出静电纺丝纳米纤维结构并评估其机械性能。最后，通过测试基于角蛋白的结构在维持人上皮细胞、大鼠神经元细胞和人原代皮肤成纤维细胞4天的细胞活力和增殖方面的功效，来分析细胞反应。
