Hao Ming, Liu Yanbo, Chen Zhijun, Hu Xiaodong, Zhang Tianyi, Zhu Xinyu, He Xingyu, Yang Bo
State Key Laboratory of New Textile Materials and Advanced Processing Technologies, School of Textile Science and Engineering, Wuhan Textile University, Wuhan 430200, China.
State Key Laboratory of Separation Membranes and Membrane Processes, School of Textile Science and Engineering, Tiangong University, Tianjin 300387, China.
Polymers (Basel). 2022 Dec 15;14(24):5505. doi: 10.3390/polym14245505.
Recently, human hair keratin has been widely studied and applied in clinical fields due to its good histocompatibility, biocompatibility, and biodegradability. However, the regenerated keratin from human hair cannot be electrospun alone because of its low molecular weight. Herein, gamma polyglutamic acid (γ-PGA) was first selected to fabricate smooth and uniform γ-PGA/keratin composite scaffolds with excellent biocompatibility and biodegradability by electrospinning technology and a chemical cross-linking method in this study. The effect of electrospinning parameters on the structure and morphology, the mechanism of chemical cross-linking, biocompatibility in vitro cell culture experiments, and biodegradability in phosphate-buffered saline buffer solution and trypsin solution of the γ-PGA/keratin electrospun nanofibrous scaffolds (ENS) was studied. The results show that the cross-linked γ-PGA/keratin ENSs had excellent water stability and biodegradability. The γ-PGA/keratin ENSs showed better biocompatibility in promoting cell adhesion and cell growth compared with the γ-PGA ENSs. It is expected that γ-PGA/keratin ENSs will be easily and significantly used in tissue engineering to repair or regenerate materials.
近年来，由于人发角蛋白具有良好的组织相容性、生物相容性和生物降解性，已在临床领域得到广泛研究和应用。然而，由于其分子量较低，人发再生角蛋白不能单独进行静电纺丝。在本研究中，首先选用γ-聚谷氨酸（γ-PGA），通过静电纺丝技术和化学交联方法制备出具有优异生物相容性和生物降解性的光滑均匀的γ-PGA/角蛋白复合支架。研究了静电纺丝参数对γ-PGA/角蛋白静电纺纳米纤维支架（ENS）的结构和形貌的影响、化学交联机理、体外细胞培养实验中的生物相容性以及在磷酸盐缓冲盐溶液和胰蛋白酶溶液中的生物降解性。结果表明，交联后的γ-PGA/角蛋白ENS具有优异的水稳定性和生物降解性。与γ-PGA ENS相比，γ-PGA/角蛋白ENS在促进细胞黏附和细胞生长方面表现出更好的生物相容性。预计γ-PGA/角蛋白ENS将易于且显著地应用于组织工程中，用于修复或再生材料。
