Dalton Martha F, Stilwell Justin M, Krimer Paula M, Miller Andrew D, Rissi Daniel R
Department of Pathology and Athens Veterinary Diagnostic Laboratory, University of Georgia College of Veterinary Medicine, Athens, GA, United States.
Section of Anatomic Pathology, Department of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, United States.
Front Vet Sci. 2019 Jul 16;6:224. doi: 10.3389/fvets.2019.00224. eCollection 2019.
The World Health Organization characterizes human choroid plexus tumor (CPT) as papilloma (CPP), atypical CPP (ACPP), and carcinoma (CPC). CPCs can disseminate via cerebrospinal fluid and be mistaken for metastatic carcinoma, creating a diagnostic challenge. Kir7.1 immunohistochemistry (IHC) is a highly reliable tool for diagnostic confirmation of CPTs and their differentiation from metastatic carcinomas in human beings and dogs. This study describes the neuropathology, Kir7.1 staining profile, and the immune cell population within the tumor microenvironment in 11 CPTs in dogs. Archived tissue sections with a diagnosis of CPT were examined and immunolabelled with Kir7.1 for diagnostic confirmation. The number of Ki67-positive neoplastic cells was calculated in 2.4 mm (equivalent to 10 FN22/40X fields), and a mean value was generated for each neoplasm. IHC for CD3, CD20, MAC387, and Iba1 was performed for immune cell characterization, and the number of stained cells for each antibody was counted in 2.4 mm, generating individual cumulative values for each antibody. -tests with Bonferroni correction evaluated IHC differences between tumor types, and Spearman's rank correlations evaluated relationships among IHC markers. Kir7.1 immunoreactivity was intense at the apical cell membrane in CPPs and ACPPs, and at the apical cell membrane and cytoplasm in CPCs. Ki67 immunoreactivity was detected in all cases. CD3+ and CD20+ lymphocytes trended together ( = 0.005) and were present within and around all CPTs. Five cases had intravascular MAC387+ monocytes. Iba1 immunoreactivity was robust within and around all tumors. Statistical differences in immune cell markers were not found among tumor types. As previously reported, Kir7.1 is a reliable antibody for the diagnosis of canine CPTs. Although immune cells were present in all cases, no significant associations were found between the type of cells and tumor diagnosis. The characterization of the immune cells within CPTs could be useful in future studies involving immunotherapy.
世界卫生组织将人类脉络丛肿瘤（CPT）分为乳头状瘤（CPP）、非典型CPP（ACPP）和癌（CPC）。CPC可通过脑脊液扩散，易被误诊为转移性癌，这给诊断带来了挑战。Kir7.1免疫组织化学（IHC）是诊断CPT及其与人类和犬类转移性癌鉴别的高度可靠工具。本研究描述了11例犬CPT的神经病理学、Kir7.1染色特征以及肿瘤微环境中的免疫细胞群体。对诊断为CPT的存档组织切片进行检查，并用Kir7.1进行免疫标记以进行诊断确认。在2.4毫米（相当于10个FN22/40X视野）中计算Ki67阳性肿瘤细胞的数量，并为每个肿瘤生成平均值。对CD3、CD20、MAC387和Iba1进行IHC以表征免疫细胞，并在2.4毫米中计数每种抗体的染色细胞数量，为每种抗体生成单独的累积值。采用Bonferroni校正的t检验评估肿瘤类型之间的IHC差异，Spearman等级相关性评估IHC标记之间的关系。Kir7.1免疫反应性在CPP和ACPP的顶端细胞膜处强烈，在CPC的顶端细胞膜和细胞质中也强烈。所有病例均检测到Ki67免疫反应性。CD3 +和CD20 +淋巴细胞呈共同趋势（P = 0.005），存在于所有CPT内部及其周围。5例病例有血管内MAC387 +单核细胞。Iba1免疫反应性在所有肿瘤内部及其周围均较强。未发现肿瘤类型之间免疫细胞标记的统计学差异。如先前报道，Kir7.1是诊断犬CPT的可靠抗体。尽管所有病例中均存在免疫细胞，但未发现细胞类型与肿瘤诊断之间存在显著关联。CPT内免疫细胞的表征可能对未来涉及免疫治疗的研究有用。
