Ryder K W, Jay S J, Glick M R, Woods J R
Department of Pathology, Indiana University School of Medicine, Wishard Memorial Hospital, Indianapolis 46202.
Clin Chem. 1988 Sep;34(9):1910-2.
Directions for pre-analytical handling of ampules of two commercially available aqueous quality-control products (contrlL and G.A.S.) contain vague instructions such as "store at room temperature" and "shake vigorously" before analysis. We examined the effect of different storage temperatures (25, 31, and 38 degrees C) and shaking rate (one, two, and four shakes per second) on pH and blood-gas results. For both products, increasing the storage temperature significantly decreased pO2 results, the magnitude of the bias being greatest for those solutions with the highest O2 tensions. However, increasing the shaking rate partly offset this bias. Increasing storage temperature also decreased results for pCO2 and increased results for pH for both manufacturers' ampules with the highest CO2 tensions, and this bias was not offset by increasing the shaking rate. We conclude that both storage temperature and shaking rate must be precisely defined and carefully monitored before these products are used in a quality-control program.
两种市售水性质量控制产品（contrlL和G.A.S.）安瓿瓶的分析前处理说明包含诸如“室温保存”和分析前“剧烈摇晃”等模糊指示。我们研究了不同储存温度（25、31和38摄氏度）和摇晃速率（每秒1次、2次和4次摇晃）对pH值和血气结果的影响。对于这两种产品，储存温度升高显著降低了pO2结果，对于那些具有最高O2张力的溶液，偏差幅度最大。然而，提高摇晃速率部分抵消了这种偏差。储存温度升高还降低了两种制造商安瓿瓶中CO2张力最高时的pCO2结果，并提高了pH值结果，且这种偏差不会因提高摇晃速率而抵消。我们得出结论，在这些产品用于质量控制程序之前，必须精确界定并仔细监测储存温度和摇晃速率。
