Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, PR China.
Anhui Key Laboratory of Modern Biomanufacturing, Hefei, Anhui 230601, PR China.
Int J Syst Evol Microbiol. 2019 Nov;69(11):3414-3419. doi: 10.1099/ijsem.0.003631.
A Gram-negative bacterium, namely strain ANRC-JHZ47, was isolated from a seawater sample collected at Biological Bay, Fildes Peninsula, Antarctica. Cells of strain ANRC-JHZ47 were rod-shaped and motile by a single polar flagellum. Strain ANRC-JHZ47 was aerobic, oxidase-negative, and catalase-positive. The strain grew at 4-37 °C (optimum, 25 °C), pH at 3.5-10.0 (optimum, pH 5.5) and in NaCl at 1-7.0 % (w/v; optimum, 2-3 %). Strain ANRC-JHZ47 used Q-8 as the predominant respiratory quinone. Its predominant fatty acids were C (21.9 %), C (12.6 %), Ccyclo 8 (12.4 %), summed feature 3 (Cω7 and/or Cω6; 13.1 %), C 3-OH (11.3 %) and summed feature 8 (Cω7 and/or Cω6; 6.0 %). Its major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified aminolipid and five unknown polar lipids. The DNA G+C content was 42.6 mol%. Strain ANRC-JHZ47 showed the highest 16S rRNA gene sequence similarity to KMM 3893 (97.9 %), followed by KMM 3633 (97.6 %), D104 (97.2 %) and IVIA-Po-185 (97.0 %). Furthermore, the average nucleotide identity values between strain ANRC-JHZ47 and KMM 3893, KMM 3633 and D104 were 79.8, 74.0, and 74.1 %, respectively. The DNA-DNA hybridization values between them were 22.5±2.5, 20.4±2.3 and 19.9±2.3 %, respectively. Based on the results of phenotypic and phylogenetic analyses, strain ANRC-JHZ47 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ANRC-JHZ47 (=MCCC 1K03604=KCTC 72113).
一株革兰氏阴性菌，即 ANRC-JHZ47 株，从南极菲尔德斯半岛生物湾采集的海水样本中分离得到。ANRC-JHZ47 株细胞呈杆状，通过单一极生鞭毛运动。该菌株为需氧、氧化酶阴性、过氧化氢酶阳性。菌株在 4-37°C（最适 25°C）、pH 3.5-10.0（最适 pH 5.5）和 NaCl 1-7.0%（w/v；最适 2-3%）的条件下生长。ANRC-JHZ47 株使用 Q-8 作为主要呼吸醌。其主要脂肪酸为 C（21.9%）、C（12.6%）、Ccyclo 8（12.4%）、summed feature 3（Cω7 和/或 Cω6；13.1%）、C 3-OH（11.3%）和 summed feature 8（Cω7 和/或 Cω6；6.0%）。其主要极性脂为磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油、一种未鉴定的胺脂和五种未知极性脂。DNA G+C 含量为 42.6mol%。ANRC-JHZ47 株与 KMM 3893（97.9%）的 16S rRNA 基因序列相似性最高，其次是 KMM 3633（97.6%）、D104（97.2%）和 IVIA-Po-185（97.0%）。此外，ANRC-JHZ47 株与 KMM 3893、KMM 3633 和 D104 的平均核苷酸同一性值分别为 79.8%、74.0%和 74.1%。它们之间的 DNA-DNA 杂交值分别为 22.5±2.5、20.4±2.3 和 19.9±2.3%。基于表型和系统发育分析的结果，ANRC-JHZ47 株被认为代表一个新的属种，建议采用新种名 。模式菌株为 ANRC-JHZ47（=MCCC 1K03604=KCTC 72113）。
