Leist T P, Heuchel R, Zinkernagel R M
Institut für Pathologie, Universitätsspital, Zürich, Switzerland.
Eur J Immunol. 1988 Aug;18(8):1295-8. doi: 10.1002/eji.1830180822.
During generalized immune responses such as the acute phase of graft-vs.-host reaction (GvHR) or many systemic viral infections the macrophage-monocyte system of mice is activated as demonstrated by their increased bactericidal capacity against Listeria monocytogenes. To study the effect of interferon (IFN)-gamma in maintainance of macrophage activity, lymphocytic choriomeningitis virus (LCMV) primed C57BL/6 mice or (C57BL/10 x B10.BR) F1 mice undergoing a GvHR were treated with polyclonal specific sheep anti-IFN-gamma antiserum. In both cases injection of anti-IFN-gamma resulted in inhibition of the stimulatory effects on macrophages as detected by reduced bactericidal activity when compared to mice treated with normal serum. Treatment with a polyclonal sheep anti-IFN-alpha,beta antiserum on the other hand did not interfere with the activation status of the macrophages. The findings suggest that IFN-gamma is produced both early in a GvHR and during the acute phase of an systemic infection with LCMV, and that it is involved in in vivo modulation of the increased activity of mononuclear phagocytes in immunologically stimulated mice.
在全身性免疫反应中，如移植物抗宿主反应（GvHR）的急性期或许多全身性病毒感染期间，小鼠的巨噬细胞-单核细胞系统会被激活，这可通过其对单核细胞增生李斯特菌杀菌能力的增强得以证明。为了研究干扰素（IFN）-γ在维持巨噬细胞活性中的作用，用多克隆特异性羊抗IFN-γ抗血清处理了经淋巴细胞性脉络丛脑膜炎病毒（LCMV）致敏的C57BL/6小鼠或正在经历GvHR的（C57BL/10×B10.BR）F1小鼠。在这两种情况下，与用正常血清处理的小鼠相比，注射抗IFN-γ导致巨噬细胞的刺激作用受到抑制，这可通过杀菌活性降低检测到。另一方面，用多克隆羊抗IFN-α、β抗血清处理并未干扰巨噬细胞的激活状态。这些发现表明，IFN-γ在GvHR早期以及LCMV全身性感染的急性期均有产生，并且它参与了免疫刺激小鼠体内单核吞噬细胞活性增强的调节。
