Department of Orthopeadics, Changhai Hospital affiliated to the Second Military Medical University, Shanghai 200433, People's Republic of China.
Orthopaedics Department, Chenggong Hospital Affilaited to Xiamen University, Xiamen 361000, People's Republic of China.
Int J Nanomedicine. 2020 Jul 14;15:5027-5042. doi: 10.2147/IJN.S241859. eCollection 2020.
Bactericidal capacity, durable inhibition of biofilm formation, and a three-dimensional (3D) porous structure are the emphases of infected bone defect (IBD) treatment via local scaffold implantation strategy.
In this study, silver nanoparticle (AgNP)-loaded nano-hydroxyapatite (nHA)@ reduced graphene oxide (RGO) 3D scaffolds (AHRG scaffolds) were designed to alleviate bone infection, inhibit biofilm formation, and promote bone repair through the synergistic effects of AgNPs, RGO, and nHA.
AHRGs were prepared using a one-step preparation method, to create a 3D porous scaffold to facilitate a uniform distribution of AgNPs and nHA. Methicillin-resistant Staphylococcus aureus (MRSA) was used as a model-resistant bacterium, and the effects of different silver loadings on the antimicrobial activity and cytocompatibility of materials were evaluated. Finally, a rabbit IBD model was used to evaluate the therapeutic effect of the AHRG scaffold in vivo.
The results showed successful synthesis of the AHRG scaffold. The ideal 3D porous structure was verified using scanning electron microscopy and transmission electron microscopy, and X-ray photoelectron spectroscopy and selected area electron diffraction measurements revealed uniform distributions of AgNP and nHA. In vitro antibacterial and cytocompatibility indicated that the 4% AHRG scaffolds possessed the most favorable balance of bactericidal properties and cytocompatibility. In vivo evaluation of the IBD model showed promising treatment efficacy of AHRG scaffolds.
The as-fabricated AHRG scaffolds effectively eliminated infection and inhibited biofilm formation. IBD repair was facilitated by the bactericidal properties and 3D porous structure of the AHRG scaffold, suggesting its potential in the treatment of IBDs.
局部支架植入策略治疗感染性骨缺损（IBD）的重点是杀菌能力、持久抑制生物膜形成和三维（3D）多孔结构。
在这项研究中，通过银纳米粒子（AgNP）负载纳米羟基磷灰石（nHA）@还原氧化石墨烯（RGO）3D 支架（AHRG 支架）的协同作用，减轻骨感染、抑制生物膜形成和促进骨修复。
采用一步法制备 AHRG，以制备 3D 多孔支架，促进 AgNP 和 nHA 的均匀分布。耐甲氧西林金黄色葡萄球菌（MRSA）被用作模型耐药菌，评估不同银载量对材料抗菌活性和细胞相容性的影响。最后，采用兔 IBD 模型评估 AHRG 支架的体内治疗效果。
结果表明成功合成了 AHRG 支架。扫描电子显微镜和透射电子显微镜验证了理想的 3D 多孔结构，X 射线光电子能谱和选区电子衍射测量表明 AgNP 和 nHA 分布均匀。体外抗菌和细胞相容性表明，4%的 AHRG 支架具有最佳的杀菌性能和细胞相容性平衡。IBD 模型的体内评价表明 AHRG 支架具有有前途的治疗效果。
所制备的 AHRG 支架有效消除感染并抑制生物膜形成。AHRG 支架的杀菌性能和 3D 多孔结构促进了 IBD 的修复，表明其在治疗 IBD 方面的潜力。
