Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, 241000, China.
Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, 241000, China.
Tissue Cell. 2019 Aug;59:10-17. doi: 10.1016/j.tice.2019.05.003. Epub 2019 May 29.
Domestic Pigeon (Columba livia) is one of few domesticated birds with an important economic value. In this study, a comprehensive investigation on the morphology and cytochemical patterns of peripheral blood cells in domestic pigeons were conducted by using wright's and various cytochemical staining techniques including periodic acid-Schiff(PAS), sudan black B(SBB), peroxidase(POX), alkaline phosphatase(ALP), acid phosphatase(ACP), chloroacetic acid AS-D naphthol esterase(AS-D) and α-naphthol acetate esterase(α-NAE) staining. Besides erythrocytes and thrombocytes, five types of leukocytes were identified: heterophils, eosinophils, basophils, lymphocytes and monocytes. Lymphocytes were the most abundant leukocytes, followed by heterophils, eosinophils, monocytes; basophils were the fewest. Erythrocytes and thrombocytes were positive for PAS, and negative for all the other cytochemical staining. Heterophils and eosinophils exhibited positive to all cytochemical staining except for α-NAE. Basophils exhibited strongly positive for POX and AS-D, positive for PAS and ALP, while negative for SBB, ACP and α-NAE staining. Monocytes exhibited positive for PAS and α-NAE, and weakly positive for ACP, while negative for SBB, POX, ALP and AS-D staining. Lymphocytes showed positive for PAS and ACP, weakly positive for AS-D, while negative for SBB, POX, ALP and α-NAE staining. Our results add up knowledge about the domestic pigeon blood cells.
家鸽（Columba livia）是少数具有重要经济价值的驯化鸟类之一。本研究采用 Wright 染色和各种细胞化学染色技术，包括过碘酸希夫（PAS）、苏丹黑 B（SBB）、过氧化物酶（POX）、碱性磷酸酶（ALP）、酸性磷酸酶（ACP）、氯乙酸 AS-D 萘酚酯酶（AS-D）和α-萘酚乙酸酯酶（α-NAE）染色，对家鸽外周血细胞的形态和细胞化学模式进行了全面研究。除红细胞和血小板外，还鉴定出 5 种白细胞：嗜中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞、淋巴细胞和单核细胞。淋巴细胞是最丰富的白细胞，其次是嗜中性粒细胞、嗜酸性粒细胞、单核细胞；嗜碱性粒细胞最少。红细胞和血小板对 PAS 呈阳性，对所有其他细胞化学染色均呈阴性。嗜中性粒细胞和嗜酸性粒细胞对所有细胞化学染色均呈阳性，除了α-NAE。嗜碱性粒细胞对 POX 和 AS-D 呈强阳性，对 PAS 和 ALP 呈阳性，对 SBB、ACP 和α-NAE 染色呈阴性。单核细胞对 PAS 和α-NAE 呈阳性，对 ACP 呈弱阳性，对 SBB、POX、ALP 和 AS-D 染色呈阴性。淋巴细胞对 PAS 和 ACP 呈阳性，对 AS-D 呈弱阳性，对 SBB、POX、ALP 和α-NAE 染色呈阴性。我们的研究结果增加了对家鸽血细胞的认识。
