Zhang Fumiao, Feng Ranran, Fang Wei, Shi Yanhui, An Liguo, Yang Guiwen
Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, China.
Anat Histol Embryol. 2019 Jan;48(1):22-32. doi: 10.1111/ahe.12407. Epub 2018 Oct 23.
Fish are the most diverse species of all vertebrate groups, and their blood cells have shown variable characteristics in terms of morphology. Cytochemical staining for enzyme activity in blood leukocytes will help assess the immune function of fish. We characterize blood cells from crucian carp (Carassius auratus) and grass carp (Ctenopharyngodon idellus) by using a Diff-Quick stain as well as different cytochemical methods. Blood specimens obtained from crucian carp and grass carp were evaluated after cytochemical staining for acid phosphatase (ACP), alkaline phosphatase (ALP), naphthol AS chloroacetate esterase (AS-DNCE), naphthyl acetate esterase (NAE), α-naphthyl butyrate esterase (NBE), peroxidase (MPO) and periodic acid-Schiff's reaction (PAS) using commercial kits. Blood cell types were evaluated based on their morphological characteristics and the presence or absence of specific chromogen. The expression pattern of enzymes was similar between the two Cyprinidae and was also broadly consistent with other fish species. However, there were some interesting differences detected between crucian carp and grass carp, including naphthol AS chloroacetate esterase activity in monocytes, peroxidase activity and location in thrombocytes. The ACP, ALP and MPO expressions of different leukocytes of the two Cyprinidae were evaluated by Image Pro Plus and were analysed for statistical significant differences. This investigation provides basic haematology and enzyme activity analyses for crucian carp and grass carp and serves as an approach to evaluating the immune response of fish.
鱼类是所有脊椎动物类群中种类最多样化的，其血细胞在形态学方面表现出多样的特征。对血液白细胞中的酶活性进行细胞化学染色将有助于评估鱼类的免疫功能。我们通过使用Diff-Quick染色法以及不同的细胞化学方法，对鲫鱼（Carassius auratus）和草鱼（Ctenopharyngodon idellus）的血细胞进行了特征描述。从鲫鱼和草鱼采集的血液标本，在使用商用试剂盒对酸性磷酸酶（ACP）、碱性磷酸酶（ALP）、萘酚AS氯乙酸酯酶（AS-DNCE）、萘乙酸酯酶（NAE）、α-萘丁酸酯酶（NBE）、过氧化物酶（MPO）和过碘酸-希夫反应（PAS）进行细胞化学染色后进行了评估。根据血细胞的形态特征以及特定色原的有无对血细胞类型进行了评估。这两种鲤科鱼类之间酶的表达模式相似，并且与其他鱼类物种也大致一致。然而，在鲫鱼和草鱼之间检测到了一些有趣的差异，包括单核细胞中的萘酚AS氯乙酸酯酶活性、血小板中的过氧化物酶活性和定位。通过Image Pro Plus对这两种鲤科鱼类不同白细胞的ACP、ALP和MPO表达进行了评估，并分析了统计学上的显著差异。本研究为鲫鱼和草鱼提供了基础血液学和酶活性分析，并作为评估鱼类免疫反应的一种方法。
