Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, China.
Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, China; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, China.
Tissue Cell. 2021 Dec;73:101633. doi: 10.1016/j.tice.2021.101633. Epub 2021 Aug 30.
The morphology, cell size and relative number of peripheral blood cells in two teleosts, Synechogobius hasta and Sebastes schlegelii, were compared using different staining methods. The results showed significant differences in cell size. The percentage of monocytes in S. hasta was greater than that in S. schlegelii (P < 0.01); however, the opposite results were obtained in the percentage of lymphocytes and thrombocytes. The two fishes shared common cytochemical-staining results, which showed that both erythrocytes were negative for all cytochemical staining; monocytes were strongly positive for PAS and positive for SBB and NAE; lymphocytes were negative for SBB, POX and NAE; neutrophils were positive for SBB and NAE; and thrombocytes were negative for SBB, ALP, POX and NAE. However, species specificity existed in the cytochemical properties. For S. hasta, monocytes were strongly positive for ALP and ACP; lymphocytes were strongly positive for ALP and weakly positive for ACP; neutrophils were strongly positive for ACP and POX; and thrombocytes were weakly positive for PAS and positive for ACP. Unlike S. hasta, monocytes were strongly positive for ACP and positive for ALP in S. schlegelii; lymphocytes were positive for ALP and partially positive for PAS; neutrophils were positive for ACP and POX; and thrombocytes were negative for PAS and ACP. The POX activity of monocytes in S. hasta was greater than that in S. schlegelii (P < 0.05), while the POX activity of neutrophils and the NAE activity of monocytes in S. hasta were significantly greater than those in S. schlegelii (P < 0.01). The results of this study can be used as a reference for the construction of haematological parameters in S. hasta and S. schlegelii for the assessment of fish health and can provide a research basis for fish diseases and immunity.
采用不同染色方法比较了两种鱼类——红娘鱼和青石斑鱼外周血细胞的形态、细胞大小和相对数量。结果表明，细胞大小存在显著差异。红娘鱼单核细胞的百分比大于青石斑鱼（P < 0.01）；然而，淋巴细胞和血小板的百分比则相反。两种鱼类具有共同的细胞化学染色结果，均显示红细胞对所有细胞化学染色均为阴性；单核细胞对 PAS 和 SBB 以及 NAE 呈强阳性；淋巴细胞对 SBB、POX 和 NAE 呈阴性；嗜中性粒细胞对 SBB 和 NAE 呈阳性；血小板对 SBB、ALP、POX 和 NAE 呈阴性。然而，在细胞化学特性方面存在种特异性。对于红娘鱼，单核细胞对 ALP 和 ACP 呈强阳性；淋巴细胞对 ALP 呈强阳性，对 ACP 呈弱阳性；嗜中性粒细胞对 ACP 和 POX 呈阳性；血小板对 PAS 呈弱阳性，对 ACP 呈阳性。与红娘鱼不同，青石斑鱼的单核细胞对 ACP 呈强阳性，对 ALP 呈阳性；淋巴细胞对 ALP 呈阳性，对 PAS 呈部分阳性；嗜中性粒细胞对 ACP 和 POX 呈阳性；血小板对 PAS 和 ACP 呈阴性。红娘鱼单核细胞的 POX 活性大于青石斑鱼（P < 0.05），而红娘鱼嗜中性粒细胞的 POX 活性和单核细胞的 NAE 活性显著大于青石斑鱼（P < 0.01）。本研究结果可为评估鱼类健康时红娘鱼和青石斑鱼血液学参数的构建提供参考，并为鱼类疾病和免疫研究提供依据。
