miRNA 106b~25簇在胃癌中的临床病理及预后意义的多因素分析
Multivariate analysis of clinicopathological and prognostic significance of miRNA 106b~25 cluster in gastric cancer.
作者信息
Li Fangxuan, Huang Jinchao, Liu Juntian, Xu Wengui, Yuan Zhiyong
机构信息
1Department of Radiotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Huanhuxi Road, Hexi District, Tianjin, 300060 China.
2Department of Cancer Prevention, Tianjin Medical University Cancer Institute and Hospital, Huanhuxi Road, Hexi District, Tianjin, 300060 China.
出版信息
Cancer Cell Int. 2019 Jul 29;19:200. doi: 10.1186/s12935-019-0918-7. eCollection 2019.
BACKGROUND
miRNA 106b25 cluster were demonstrated to be an oncogene. In previous study, we had analyzed the diagnostic significance of miRNA 106b25 based on its carcinogenesis effect. The significance of miRNA 106b~25 for prognosis of gastric cancer were not researched.
METHODS
We applied multivariate analysis of PCA, PLS-DA and Cox Regression for clinicopathological features and survival time to explore the significance of miRNA 106b~25 expression in plasma and cancer tissues for gastric cancer.
RESULTS
The expression of miRNA 106b, miRNA 93 and miRNA 25 in plasma were positively correlated with their expression in tumor tissues. Via PCA analysis, it was found that miRNA 106b25 expression in plasma and tumor, T, N and TNM stage were correlated with each other. Via PLS-DA analysis, we identified that T, N and TNM stage were important factors for miRNA 106b25 expression both in plasma and tumor (all VIP value > 1.2). According to loading weights of variables for the first and second components, it was found that the importance of the miRNA 106b~25s expression carried with the progressed stage of gastric cancer. In the survival analysis, COX regression showed that T stage, plasma miRNA 106b and tumor miRNA 93 were significant risk factors for overall survival [HR: 0.400 (0.205-0.780); = 0.007; HR: 0.371 (0.142-0.969), = 0.043; 0.295 (0.134-0.650), = 0.002].
CONCLUSION
Plasma and tumor miRNA 106b25 expression correlated with T, N and TNM stage. Increased miRNA 106b25 expression was important characters carried with gastric cancer progression. T stage, plasma miRNA106b and tumor miRNA 93 significant risk factors for overall survival.
背景
miRNA 106b25簇被证明是一种癌基因。在先前的研究中，我们基于其致癌作用分析了miRNA 106b25的诊断意义。但尚未研究miRNA 106b~25对胃癌预后的意义。
方法
我们应用主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）和Cox回归对临床病理特征和生存时间进行多变量分析，以探讨血浆和癌组织中miRNA 106b~25表达对胃癌的意义。
结果
血浆中miRNA 106b、miRNA 93和miRNA 25的表达与其在肿瘤组织中的表达呈正相关。通过PCA分析发现，血浆和肿瘤中miRNA 106b25的表达与T、N和TNM分期相互关联。通过PLS-DA分析，我们确定T、N和TNM分期是血浆和肿瘤中miRNA 106b25表达的重要因素（所有VIP值>1.2）。根据第一和第二成分的变量载荷权重，发现miRNA 106b~25表达的重要性与胃癌进展阶段相关。在生存分析中，COX回归显示T分期、血浆miRNA 106b和肿瘤miRNA 93是总生存的显著危险因素[风险比（HR）：0.400（0.205 - 0.780）；P = 0.007；HR：0.371（0.142 - 0.969），P = 0.043；0.295（0.134 - 0.650），P = 0.002]。
结论
血浆和肿瘤中miRNA 106b25的表达与T、N和TNM分期相关。miRNA 106b25表达增加是胃癌进展的重要特征。T分期、血浆miRNA106b和肿瘤miRNA 93是总生存的显著危险因素。
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