Sananta Panji, Jonatan Andrew, Ernanda Shelby Amrus, Kartikaningtyas Ayu Novita, Parhusip Yanti Marito, Amelia Yesi, Maulidya Elli, Juwono Muthi'ah Adira
Orthopedic Department, Faculty of Medicine, Brawijaya University, Indonesia.
Bio Medics, Faculty of Medicine, Brawijaya University, Indonesia.
Pan Afr Med J. 2019 May 17;33:37. doi: 10.11604/pamj.2019.33.37.17606. eCollection 2019.
Osteoporosis is characterized by low bone mass and density, as well as change in microarchitecture of bone tissue leading to decreased bone strength. In vitro research shows nicotine can increase osteoblast activity and proliferation, also suppress osteoclast activity. Therefore we explore nicotine anti-resorptive property by in vivo true experimental and randomized posttest only controlled group research that was conducted in 18-20 weeks old .
Twenty-five female rats were divided into five groups, with 5 rats per group. The first group represented normal rats (Sham), while the second to fifth group underwent bilateral ovariectomy. The second group serves as positive control group (ovariectomy-only/OVX). The third to fifth group serve as dose 1 (P1-0.25mg/kg), dose 2 (P2-0.5 mg/kg), and Dose 3 (P3-0.75 mg/kg) treatment group receiving daily per-oral nicotine for 28 days, started 3 weeks post- ovariectomy. After 28 days treatment, the serum was checked.
Nicotine has dose-dependent manner on serum osteocalcin and serum DPD level. Level of osteocalcin in P2 group was significantly lower (Mann-Whitney, p = 0.008) compared to OVX group (59.4% lower). Level of DPD in all group was not significantly different (ANOVA, p < 0.05) but shows lowest level in P2 group. For serum calcitonin level, there's no significant different between groups.
Nicotine at right low-dose might be able to inhibit osteoclast activity, thus open a possibility of anti-resorptive property of nicotine.
骨质疏松症的特征是骨量和骨密度降低，以及骨组织微结构改变导致骨强度下降。体外研究表明，尼古丁可增加成骨细胞活性和增殖，还可抑制破骨细胞活性。因此，我们通过在18 - 20周龄大鼠中进行的体内真实实验和随机后测单对照组研究，探讨尼古丁的抗吸收特性。
将25只雌性大鼠分为五组，每组5只。第一组为正常大鼠（假手术组），而第二至五组进行双侧卵巢切除术。第二组作为阳性对照组（仅卵巢切除组/OVX）。第三至五组作为剂量1（P1 - 0.25mg/kg）、剂量2（P2 - 0.5mg/kg）和剂量3（P3 - 0.75mg/kg）治疗组，在卵巢切除术后3周开始每天经口给予尼古丁，持续28天。治疗28天后，检查血清。
尼古丁对血清骨钙素和血清DPD水平有剂量依赖性影响。与OVX组相比，P2组骨钙素水平显著降低（曼-惠特尼检验，p = 0.008）（降低59.4%）。所有组的DPD水平无显著差异（方差分析，p < 0.05），但P2组显示最低水平。对于血清降钙素水平，各组之间无显著差异。
低剂量的尼古丁可能能够抑制破骨细胞活性，从而开启尼古丁具有抗吸收特性的可能性。
