Hashemi Sayyed Hossein, Kaykhaii Massoud, Jamali Keikha Ahmad, Mirmoradzehi Elahe, Sargazi Ghasem
1Department of Marine Chemistry, Faculty of Marine Science, Chabahar Maritime University, Chabahar, Iran.
2Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan, 98155-674 Iran.
BMC Chem. 2019 Apr 23;13(1):59. doi: 10.1186/s13065-019-0572-0. eCollection 2019 Dec.
This paper describes the application of response surface methodology (RSM) to develop a miniaturized metal organic framework based pipette-tip solid phase extraction for the extraction of malachite green (MG), rhodamine B (RB), methyl orange (MO) and acid red 18 (AR) dyes from seawater samples and their determination by high performance liquid chromatography. The effects of various parameters such as pH of the sample solution, type and amount of added salt, type and volume of eluent solvent, concentration of surfactant (triton X-114), sample volume, and number of cycles of extraction and desorption were investigated and optimized by two methods of one-variable-at-a-time and RSM based on Box-Behnken design. Under optimum conditions, the linear range of the method was 0.5-200.0 µg/L for RB and MG and 1.0-150.0 µg/L for AR and MO. Limits of detection of the analytes were obtained in the range of 0.09-0.38 µg/L. Reproducibility of the method (as RSD %) was better than 6.4%. The method has been successfully used for analysis of four dyes in seawater of Chabahar Bay.
本文描述了响应面法（RSM）在开发基于小型化金属有机框架的移液器吸头固相萃取中的应用，用于从海水样品中萃取孔雀石绿（MG）、罗丹明B（RB）、甲基橙（MO）和酸性红18（AR）染料，并通过高效液相色谱法对其进行测定。通过单因素法和基于Box-Behnken设计的响应面法研究并优化了各种参数的影响，如样品溶液的pH值、添加盐的类型和量、洗脱溶剂的类型和体积、表面活性剂（曲拉通X-114）的浓度、样品体积以及萃取和解吸的循环次数。在最佳条件下，该方法对RB和MG的线性范围为0.5 - 200.0 μg/L，对AR和MO的线性范围为1.0 - 150.0 μg/L。分析物的检测限在0.09 - 0.38 μg/L范围内。该方法的重现性（以相对标准偏差计）优于6.4%。该方法已成功用于恰巴哈尔湾海水中四种染料的分析。
