Ghasemi Elham, Kaykhaii Massoud
Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan 98135-674, Iran.
Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan 98135-674, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Jul 5;164:93-7. doi: 10.1016/j.saa.2016.04.001. Epub 2016 Apr 9.
A novel, green, simple and fast method was developed for spectrophotometric determination of Malachite green, Crystal violet, and Rhodamine B in water samples based on Micro-cloud Point extraction (MCPE) at room temperature. This is the first report on the application of MCPE on dyes. In this method, to reach the cloud point at room temperature, the MCPE procedure was carried out in brine using Triton X-114 as a non-ionic surfactant. The factors influencing the extraction efficiency were investigated and optimized. Under the optimized condition, calibration curves were found to be linear in the concentration range of 0.06-0.60mg/L, 0.10-0.80mg/L, and 0.03-0.30mg/L with the enrichment factors of 29.26, 85.47 and 28.36, respectively for Malachite green, Crystal violet, and Rhodamine B. Limit of detections were between 2.2 and 5.1μg/L.
基于室温下的微浊点萃取（MCPE），开发了一种新颖、绿色、简便且快速的分光光度法，用于测定水样中的孔雀石绿、结晶紫和罗丹明B。这是关于MCPE在染料应用方面的首次报道。在该方法中，为了在室温下达到浊点，使用Triton X-114作为非离子表面活性剂，在盐水中进行MCPE过程。研究并优化了影响萃取效率的因素。在优化条件下，发现孔雀石绿、结晶紫和罗丹明B的校准曲线在浓度范围0.06 - 0.60mg/L、0.10 - 0.80mg/L和0.03 - 0.30mg/L内呈线性，富集因子分别为29.26、85.47和28.36。检测限在2.2至5.1μg/L之间。
