Rai Pawankumar, Mehrotra Srishti, Lahane Vaibhavi, Yadav Akhilesh K, Sharma Sandeep K
Food Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh 226001 India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India.
J Food Sci Technol. 2025 Jan;62(1):65-74. doi: 10.1007/s13197-024-06010-3. Epub 2024 Jun 4.
Solid phase extraction technique is a widely used sample preparation technique for the extraction of components from complex food matrices. However, there are several parameters in SPE that leads to low recovery, and reproducibility, insufficiently clean extracts and evaporation of volatile compounds. These drawbacks can be addressed through the use of innovative techniques and instrumentation that offers improved efficiency and accuracy for isolation of active constituents from food and beverage samples. Here a solid phase extraction methodology and extraction assembly has been developed for extracting compounds from orange fruit juice, followed by analysis using a LC-HRMS system. The methodology and assembly eliminate the evaporation step, thus facilitating the analysis of wide range of volatile and non-volatile compounds without the risk of their evaporation, making it particularly suitable for thermo-sensitive compounds. Also, the elute can be used for further analysis using LC-HRMS. The effectiveness of the designed extraction assembly and technique was demonstrated by comparing the procedure with the available conventional extraction assemblies, highlighting the advantages of the developed method.
The online version contains supplementary material available at 10.1007/s13197-024-06010-3.
固相萃取技术是一种广泛应用的样品制备技术，用于从复杂的食品基质中提取成分。然而，固相萃取中有几个参数会导致回收率低、重现性差、提取物不够纯净以及挥发性化合物蒸发。这些缺点可以通过使用创新技术和仪器来解决，这些技术和仪器能提高从食品和饮料样品中分离活性成分的效率和准确性。本文开发了一种固相萃取方法和萃取装置，用于从橙汁中提取化合物，随后使用液相色谱-高分辨率质谱系统进行分析。该方法和装置省去了蒸发步骤，从而便于分析各种挥发性和非挥发性化合物，而不会有蒸发风险，使其特别适用于热敏性化合物。此外，洗脱液可用于液相色谱-高分辨率质谱的进一步分析。通过将该方法与现有的传统萃取装置进行比较，证明了所设计的萃取装置和技术的有效性，突出了所开发方法的优点。
在线版本包含可在10.1007/s13197-024-06010-3获取的补充材料。
