Cui Lili, Hou Xingzi, Li Wenjing, Leng Yuchun, Zhang Yang, Li Xinjuan, Hou Yangyang, Liu Zhenhua, Kang Wenyi
1National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 China.
Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng, 475004 Henan China.
BMC Chem. 2019 Jul 9;13(1):81. doi: 10.1186/s13065-019-0602-y. eCollection 2019 Dec.
The dynamic changes of secondary metabolites and tyrosinase activity of flowers were investigated during blooming for full use of flowers. Phlorizin, astragalin and afzelin were selected as the indicators to determine the optimum extraction conditions of flowers by HPLC analysis and three-factor and three-level orthogonal design experiments. When the smashing mesh number was 40 mesh, the ultrasonic time was 30 min, the solid-liquid ratio was 1:120, and the centrifugal speed was 10,000 r/min, the total content of the three compounds was the highest. The total content of phlorizin, astragalin and afzelin increased firstly and then decreased, and the highest was on the third day (176.74 mg/g). At the same time, the tyrosinase activity of flowers showed that it had an activation effect on tyrosinase during early blooming period, however it exhibited inhibitory effect during late blooming period.
为充分利用花朵，研究了花朵在开花期间次生代谢产物和酪氨酸酶活性的动态变化。选择根皮苷、黄芪苷和花旗松素作为指标，通过高效液相色谱分析和三因素三水平正交设计实验确定花朵的最佳提取条件。当粉碎目数为40目、超声时间为30分钟、固液比为1:120、离心速度为10000转/分钟时，三种化合物的总含量最高。根皮苷、黄芪苷和花旗松素的总含量先升高后降低，最高值出现在第三天（176.74毫克/克）。同时，花朵的酪氨酸酶活性表明，其在开花初期对酪氨酸酶有激活作用，而在开花后期表现出抑制作用。
