衣原体的正向和反向遗传学分析
Forward and Reverse Genetic Analysis of Chlamydia.
作者信息
Kędzior Mateusz, Bastidas Robert J
机构信息
Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA.
出版信息
Methods Mol Biol. 2019;2042:185-204. doi: 10.1007/978-1-4939-9694-0_13.
Abstract
Chlamydia is a major etiological agent of human disease that affects millions of individuals worldwide. Historically, our understanding of the mechanisms that contribute to its pathogenesis has been limited. However, the recent development of powerful genetic tools for manipulating Chlamydia has resulted in significant gains in our ability to dissect its virulence mechanisms. These tools have overcome several barriers for manipulating intracellular pathogens and are amenable for the routine genetic engineering of Chlamydia. Here, we provide several detailed protocols for performing genetic analysis in Chlamydia trachomatis allowing investigators to elucidate how this obligate intracellular pathogen causes disease.
摘要
衣原体是一种主要的人类疾病病原体，影响着全球数百万人。从历史上看，我们对其发病机制的理解一直有限。然而，最近用于操纵衣原体的强大遗传工具的发展，使我们剖析其毒力机制的能力有了显著提高。这些工具克服了操纵细胞内病原体的几个障碍，适用于衣原体的常规基因工程。在这里，我们提供了几个详细的方案，用于在沙眼衣原体中进行遗传分析，使研究人员能够阐明这种专性细胞内病原体是如何引起疾病的。
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