Costas Malvido Mónica, Alonso González Elisa, Outeiriño David, Pérez Guerra Nelson
1Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004 Ourense, Spain.
2Department of Chemical Engineering, Faculty of Sciences, University of Vigo, Ourense Campus, As Lagoas s/n, 32004 Ourense, Spain.
3 Biotech. 2018 Jul;8(7):292. doi: 10.1007/s13205-018-1318-8. Epub 2018 Jun 25.
In this study, probiotic biomass and nisin productions by CECT 539 were followed in two realkalized fed-batch cultures in diluted whey (DW) supplemented with KHPO up to a total phosphorus concentration of 0.459 g/L. Increased biomass (5.12 g/L, 2.18 × 10 CFU/mL) and nisin (235.23 BU/mL) concentrations were obtained in the culture fed concentrated whey and concentrated mussel processing waste (CMPW) medium supplemented with glucose up to a concentration of 400 g/L (CMPW + G medium) compared with similar fed-batch fermentations in DW medium. In the second fed-batch fermentation, the feeding medium CMPW + G was supplemented with KHPO up to a TP concentration of 3.21 g/L. With this approach, increased production of biomass (5.49 g/L, 2.33 × 10 CFU/mL) and nisin (257.59 BU/mL) was obtained. Considering the substantial availability of these wastes at very low prices from local dairy and mussel processing plants in Galicia, their use as culture media could offer an attractive alternative for a low-cost production of probiotic biomass and nisin at a high scale.
在本研究中，在两种重新碱化的补料分批培养中，跟踪了CECT 539产生的益生菌生物量和乳酸链球菌素，培养采用稀释乳清（DW），添加KHPO直至总磷浓度达到0.459 g/L。与在DW培养基中进行的类似补料分批发酵相比，在补料分批培养中，向浓缩乳清和浓缩贻贝加工废料（CMPW）培养基中添加浓度高达400 g/L的葡萄糖（CMPW + G培养基），可提高生物量（5.12 g/L，2.18×10 CFU/mL）和乳酸链球菌素（235.23 BU/mL）的浓度。在第二次补料分批发酵中，向补料培养基CMPW + G中添加KHPO直至总磷浓度达到3.21 g/L。通过这种方法，生物量（5.49 g/L，2.33×10 CFU/mL）和乳酸链球菌素（257.59 BU/mL）的产量增加。考虑到加利西亚当地乳制品厂和贻贝加工厂以极低价格大量供应这些废料，将它们用作培养基可为大规模低成本生产益生菌生物量和乳酸链球菌素提供有吸引力的替代方案。
