松柏醇通过调节基质金属蛋白酶-2 表达和细胞外信号调节激酶通路减少口腔癌细胞的迁移和侵袭。
Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway.
机构信息
Department of Otorhinolaryngology, Head and Neck Surgery, Changhua Christian Hospital, Changhua 500, Taiwan.
Department of Family Medicine, Changhua Christian Hospital, Changhua 500, Taiwan; Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Department of Holistic Wellness, Mingdao University, Changhua 52345, Taiwan.
出版信息
Biomed Pharmacother. 2019 Sep;117:109160. doi: 10.1016/j.biopha.2019.109160. Epub 2019 Jul 1.
BACKGROUND
Pinosylvin possesses several biological properties, including anti-inflammatory, antitumor, and antioxidant characteristics. However, the effects of pinosylvin on the migration and invasion of human oral cancer cells and the underlying mechanisms remain unclear.
HYPOTHESIS/PURPOSE: In this research, we investigated the outcome of different concentrations of pinosylvin (0-80 μM) on the metastatic and invasive abilities of SAS, SCC-9, and HSC-3 cells.
METHODS AND RESULTS
Western blotting assay and Gelatin zymography assay indicated that pinosylvin inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and reduced its protein level but increased the expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). Additionally, the wound healing assay and Transwell method showed that pinosylvin reduced the migration of SAS, SCC-9 and HSC-3 oral cancer cells. Besides, pinosylvin decreased the phosphorylation of ERK1/2 protein experssion in both SAS and SCC-9 cells.
CONCLUSION
These results indicate that pinosylvin is a potential anticancer agent for preventing oral cancer metastasis.
背景
松脂素具有多种生物学特性，包括抗炎、抗肿瘤和抗氧化特性。然而，松脂素对人口腔癌细胞迁移和侵袭的影响及其潜在机制尚不清楚。
假设/目的：本研究旨在探讨不同浓度的松脂素（0-80 μM）对 SAS、SCC-9 和 HSC-3 细胞转移和侵袭能力的影响。
方法和结果
Western blot 分析和明胶酶谱分析表明，松脂素抑制基质金属蛋白酶-2（MMP-2）的酶活性，降低其蛋白水平，增加组织金属蛋白酶抑制剂-2（TIMP-2）的表达。此外，划痕愈合实验和 Transwell 法表明，松脂素减少了 SAS、SCC-9 和 HSC-3 口腔癌细胞的迁移。此外，松脂素降低了 SAS 和 SCC-9 细胞中 ERK1/2 蛋白表达的磷酸化。
结论
这些结果表明，松脂素是一种预防口腔癌转移的潜在抗癌药物。
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