School of Veterinary Medicine, Veterinary Science Centre, University College Dublin, Belfield, Dublin 4, Ireland.
School of Agriculture and Food Science, University College Dublin, Lyons Research Farm, Celbridge, Co. Kildare W23 ENY2, Ireland.
Mar Drugs. 2019 Aug 5;17(8):457. doi: 10.3390/md17080457.
Fucose sulphated polysaccharides (FSPs) and glucans have recently attracted the attention of the scientific community due to their wide range of biological activities. Both polysaccharides should ideally be selectively extracted using innovative technologies with high extraction efficiency. This study aims to: (1) Optimise the extraction variables used in hydrothermal-assisted extraction (HAE) to obtain high yields of FSPs, total glucans, and antioxidants from ; (2) to apply these optimised protocols to other brown macroalgae; and (3) to explore the application of ultrasound and thermal technologies to increase the recovery of polysaccharides from the residual biomass. Box-Behnken design (three-factor, four-levels) was employed to optimise the HAE variables, and principal component analysis was used to evaluate the recovery of polysaccharides from the residual biomass. The optimal HAE conditions were 120 °C, 80.9 min, and 12.02 mL/g macroalgae from . The best sequential application of ultrasound and thermal treatment achieved an additional 2971.7 ± 61.9 mg fucose/100 g dried macroalgal residue (dmr) from and 908.0 ± 51.4 mg total glucans/100 g dmr from macroalgal residues.
硫酸化岩藻糖多糖（FSPs）和葡聚糖由于其广泛的生物活性而引起了科学界的关注。这两种多糖都应使用具有高效提取率的创新技术选择性提取。本研究旨在：（1）优化水热辅助提取（HAE）中使用的提取变量，以从 中获得高产量的 FSPs、总葡聚糖和抗氧化剂；（2）将这些优化的方案应用于其他褐藻；（3）探索超声和热技术的应用，以提高从剩余生物质中回收多糖的效率。采用 Box-Behnken 设计（三因素、四水平）优化 HAE 变量，并采用主成分分析评估从剩余生物质中回收多糖的效果。从 中提取 FSPs、总葡聚糖和抗氧化剂的最佳 HAE 条件为 120°C、80.9 min 和 12.02 mL/g 海藻。超声和热处理的最佳连续应用从 中的剩余海藻残渣中额外获得了 2971.7 ± 61.9 mg 岩藻糖/100 g 干燥海藻残渣（dmr），从 中的剩余海藻残渣中获得了 908.0 ± 51.4 mg 总葡聚糖/100 g dmr。
