School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
Int J Mol Sci. 2019 Aug 5;20(15):3816. doi: 10.3390/ijms20153816.
Inflammatory bowel disease (IBD) is a worldwide healthcare problem calling for the development of new therapeutic drugs. and Roscoe are two common dietetic Chinese herbs, which are traditionally used for complementary treatment of gastrointestinal disorders. As bioactive constituents, volatile and pungent substances of these two herbs could be effectively extracted together by supercritical fluid extraction. In this study, the supercritical fluid extract of and Roscoe (AZ-SFE) was obtained by an optimized extraction process and it was chemically characterized. The anti-inflammatory effect and underlying mechanism of AZ-SFE were evaluated in a lipopolysaccharide (LPS)-induced RAW264.7 cell model and a 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis rat model. AZ-SFE notably inhibited the production of NO in LPS-stimulated macrophages, and it inhibited the proliferation of Concanavalin A (Con A)-induced splenocytes with suppression of the Th1 immune response. In vivo, the study demonstrated that AZ-SFE significantly alleviated disease activity, colonic shortening, macroscopic damage and histological injury of TNBS-treated rats with reduction of oxidative stress, suppression of inflammatory cytokines, and modulation of hepcidin and serum iron. These findings suggested that AZ-SFE may be a promising supplement for current IBD therapy.
炎症性肠病（IBD）是一个全球性的医疗保健问题，需要开发新的治疗药物。当归和川芎是两种常见的药用中药，传统上用于治疗胃肠道疾病的辅助治疗。作为生物活性成分，这两种草药的挥发性和刺激性物质可以通过超临界流体萃取有效地一起提取。在这项研究中，通过优化的提取工艺获得了当归和川芎的超临界流体提取物（AZ-SFE），并对其进行了化学表征。在脂多糖（LPS）诱导的 RAW264.7 细胞模型和 2,4,6-三硝基苯磺酸（TNBS）诱导的结肠炎大鼠模型中评估了 AZ-SFE 的抗炎作用及其潜在机制。AZ-SFE 显著抑制 LPS 刺激的巨噬细胞中 NO 的产生，并抑制刀豆蛋白 A（Con A）诱导的脾细胞增殖，抑制 Th1 免疫反应。在体内，研究表明 AZ-SFE 可显著减轻 TNBS 处理大鼠的疾病活动、结肠缩短、宏观损伤和组织学损伤，减轻氧化应激、抑制炎症细胞因子，并调节铁调素和血清铁。这些发现表明，AZ-SFE 可能是当前 IBD 治疗的一种有前途的补充。
