Zhao Bochen, Kang Qian, Peng Yu, Xie Yuanping, Chen Cheng, Li Bingshao, Wu Qing
School of Chinese Material Medica, Beijing University of Chinese Medicine, No. 6, Wangjing Zhonghuan Nanlu, Beijing 100102, China.
Int J Mol Sci. 2017 Aug 11;18(8):1750. doi: 10.3390/ijms18081750.
root (ASR) extract was obtained to investigate its effects on colorectal carcinogenesis in different stages of an Azoxymethane/Dextran sodium sulphate (AOM/DSS) model. In this study, we showed that ASR extract administration in the initial stage of the AOM/DSS model had cancer preventive effects with decreasing tumor incidence and a high-grade of intraepithelial neoplasia incidence. With respect to DNA damage, the amounts of 8-oxoguanine and γ-H2AX were suppressed in colon tissue. The balance of apoptosis and proliferation was approaching the normal state. In contrast, ASR extract administration in the promotion stage of the AOM/DSS model accelerated the progression of carcinogenesis. The maximum tumor size reached 49.85 ± 25.04 mm³. High-grade pathological changes were significantly increased. Decreased DNA damage and P53 level reflected the disrupted reactive oxygen species (ROS) concentration in colorectal tissue, which led to an imbalance of proliferative and apoptotic relationships. These findings suggested that the cancer-preventive effect of ASR extract may be stage-dependent in the process of carcinogenesis.
提取了根（ASR）提取物，以研究其在偶氮甲烷/葡聚糖硫酸钠（AOM/DSS）模型不同阶段对结直肠癌发生的影响。在本研究中，我们发现，在AOM/DSS模型的初始阶段给予ASR提取物具有癌症预防作用，可降低肿瘤发生率和高级别上皮内瘤变发生率。关于DNA损伤，结肠组织中8-氧代鸟嘌呤和γ-H2AX的量受到抑制。细胞凋亡与增殖的平衡接近正常状态。相比之下，在AOM/DSS模型的促进阶段给予ASR提取物会加速致癌进程。最大肿瘤大小达到49.85±25.04立方毫米。高级别病理变化显著增加。DNA损伤和P53水平降低反映了结直肠组织中活性氧（ROS）浓度紊乱，导致增殖与凋亡关系失衡。这些发现表明，ASR提取物的癌症预防作用在致癌过程中可能具有阶段依赖性。
