Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo Leon, Av. Francisco I. Madero S/N, Mitras Centro, Monterrey, Nuevo Leon 64460, Mexico.
Departamento de Ciencias Básicas. Escuela de Medicina, Universidad de Monterrey, Av. Morones Prieto No. 4500 pte, San Pedro Garza García, Nuevo Leon 64238, Mexico.
Viruses. 2019 Aug 5;11(8):714. doi: 10.3390/v11080714.
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes Chikungunya fever. CHIKV entered Mexico through the state of Chiapas in October 2014. To fully understand the Chikungunya fever outbreak that occurred in southern Chiapas during 2015, we evaluated 22 PCR-confirmed CHIKV-positive patients, identified CHIKV genetic variability, reconstructed viral dispersal, and assessed possible viral mutations. Viruses were isolated and , , and genes were sequenced. We applied phylogenetic and phylogeographic approaches, modeled mutations, and estimated selective pressure. Different CHIKV strains circulated in Chiapas during summer 2015. Three isolates grouped themselves in a well-supported clade. Estimates show that the outbreak started in Ciudad Hidalgo and posteriorly dispersed towards Tapachula and neighboring municipalities. We found six non-synonymous mutations in our isolates. Two mutations occurred in one isolate and the remaining mutations occurred in single isolates. Mutations E2 T116I and E2 K221R changed the protein surface in contact with the host cell receptors. We could not find positive selected sites in our CHIKV sequences from southern Chiapas. This is the first viral phylogeographic reconstruction in Mexico characterizing the CHIKV outbreak in southern Chiapas.
基孔肯雅热病毒（CHIKV）是一种虫媒黄病毒，可引起基孔肯雅热。2014 年 10 月，CHIKV 通过恰帕斯州进入墨西哥。为了全面了解 2015 年恰帕斯南部发生的基孔肯雅热疫情，我们评估了 22 例经 PCR 确认的 CHIKV 阳性患者，确定了 CHIKV 遗传变异性，重建了病毒传播，并评估了可能的病毒突变。分离并测序了病毒的 、 和 基因。我们应用了系统发育和系统地理学方法、建模突变并估计了选择压力。2015 年夏季恰帕斯州有不同的 CHIKV 株系传播。3 个分离株聚集在一个支持度较高的分支中。估计显示，疫情始于 Ciudad Hidalgo，随后向 Tapachula 和邻近的市镇传播。我们在分离株中发现了 6 个非同义突变。其中 2 个突变发生在一个分离株中，其余的突变发生在单个分离株中。E2 蛋白上的 T116I 和 K221R 突变改变了与宿主细胞受体相互作用的蛋白表面。我们在恰帕斯南部的 CHIKV 序列中没有发现阳性选择位点。这是首次在墨西哥进行的病毒系统地理学重建，描述了恰帕斯南部的 CHIKV 疫情。
