Hazawa Masaharu, Lim Kee Siang, Dewi Firli R P, Kobayashi Akiko, Wong Richard W
1School of Natural System, Institute of Natural Science and Technology, Kanazawa University, Kanazawa, Japan.
2Biology Department, Faculty of Mathematics and Natural Science, Universitas Negeri Makassar, Jl. Dg Tata Raya, Makassar, 90224 Indonesia.
Cell Div. 2019 Aug 3;14:7. doi: 10.1186/s13008-019-0050-z. eCollection 2019.
Nuclear pore complexes (NPCs) act as nano-turnstiles within nuclear membranes between the cytoplasm and nucleus of mammalian cells. NPC proteins, called nucleoporins (Nups), mediate trafficking of proteins and RNA into and out of the nucleus, and are involved in a variety of mitotic processes. We previously reported that Nup62 localizes to the centrosome and mitotic spindle during mitosis, and plays a role in centrosome homeostasis. However, whether Nup58, a Nup62 subcomplex protein, also localizes to spindle poles is unknown.
Herein, we show that Nup58 localizes to the nuclear rim during interphase, and to mitotic spindles, centrosomes, and midbodies during mitosis. Our confocal microscopy, live-cell imaging, and stimulated emission depletion nanoscopy results also demonstrated that Nup58 localized to the centrosomes during metaphase and relocalized to midbodies during abscission. Depletion of Nup58 resulted in centrosomal abnormalities and delayed abscission.
Nup58 localized at the centrosomes and mitotic spindle during metaphase and relocalized at midbodies during abscission. This study highlights the important role of Nup58 in mitosis.
核孔复合体（NPCs）在哺乳动物细胞质与细胞核之间的核膜内充当纳米旋转门。核孔复合体蛋白，即核孔蛋白（Nups），介导蛋白质和RNA进出细胞核的运输，并参与多种有丝分裂过程。我们之前报道过，Nup62在有丝分裂期间定位于中心体和有丝分裂纺锤体，并在中心体稳态中发挥作用。然而，作为Nup62亚复合体蛋白的Nup58是否也定位于纺锤体极尚不清楚。
在此，我们表明Nup58在间期定位于核边缘，在有丝分裂期间定位于有丝分裂纺锤体、中心体和中间体。我们的共聚焦显微镜、活细胞成像和受激发射损耗纳米显微镜结果还表明，Nup58在中期定位于中心体，并在胞质分裂期间重新定位于中间体。Nup58的缺失导致中心体异常和胞质分裂延迟。
Nup58在中期定位于中心体和有丝分裂纺锤体，并在胞质分裂期间重新定位于中间体。本研究突出了Nup58在有丝分裂中的重要作用。
