State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Shandong Provincial Key Laboratory of Vegetable Disease and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, China.
Planta. 2019 Nov;250(5):1603-1612. doi: 10.1007/s00425-019-03238-8. Epub 2019 Aug 6.
A rice allele of PSKR1 functioning in resistance to bacterial leaf streak was identified. Phytosulfokine (PSK), a disulfated pentapeptide encoded by precursor genes that are ubiquitously present in higher plants, belongs to the group of plant peptide growth factors. The PSK receptor PSKR1 in Arabidopsis thaliana is an active kinase and has guanylate cyclase activity resulting in dual-signaling outputs. Here, the LOC_Os02g41890 out of three candidates completely rescued root growth and susceptible to Pseudomonas syringae pv. DC3000 in the Arabidopsis pskr1-3 mutant and was identified as OsPSKR1. This protein was localized to plasma membrane similar to AtPSKR1. The expression of OsPSKR1 was upregulated upon inoculation with RS105, a strain of Xanthomonas oryzae pv. oryzicola (Xoc) that cause bacterial leaf streak in rice. OsPSKR1 overexpression (OE) lines had greater resistance to RS105 than the wild type. Consistently, the expression of pathogenesis-related genes involved in the salicylic acid (SA) pathway was upregulated in the transgenic lines. Overall, OsPSKR1 functions as a candidate PSK receptor and regulates resistance to Xoc by activating the expression of pathogenesis-related genes involved in the SA pathway in rice.
一个在抗细菌性条斑病中起作用的水稻 PSKR1 等位基因被鉴定出来。植物磺基肽（PSK）是一种由前体基因编码的二硫酸五肽，广泛存在于高等植物中，属于植物肽生长因子群。拟南芥中的 PSK 受体 PSKR1 是一种活性激酶，具有鸟苷酸环化酶活性，从而产生双重信号输出。在这里，三个候选基因中的 LOC_Os02g41890 完全挽救了拟南芥 pskr1-3 突变体中的根生长，并对丁香假单胞菌 pv.DC3000 敏感，被鉴定为 OsPSKR1。该蛋白与 AtPSKR1 相似，定位于质膜。OsPSKR1 的表达在接种 RS105（一种引起水稻细菌性条斑病的稻黄单胞菌 pv.oryzicola（Xoc）菌株）时上调。与野生型相比，OsPSKR1 过表达（OE）系对 RS105 的抗性更强。一致地，与水杨酸（SA）途径相关的发病相关基因的表达在转基因系中上调。总的来说，OsPSKR1 作为候选 PSK 受体发挥作用，通过激活与 SA 途径相关的发病相关基因的表达来调节水稻对 Xoc 的抗性。
