脑类器官作为人类神经发育障碍建模的工具。
Brain Organoids as Tools for Modeling Human Neurodevelopmental Disorders.
机构信息
Department of Pediatrics/Rady Children's Hospital San Diego, School of Medicine, University of California San Diego, San Diego, California.
Department of Cellular & Molecular Medicine, Stem Cell Program, Center for Academic Research and Training in Anthropogeny (CARTA), Kavli Institute for Brain and Mind, La Jolla, California.
出版信息
Physiology (Bethesda). 2019 Sep 1;34(5):365-375. doi: 10.1152/physiol.00005.2019.
Abstract
Brain organoids recapitulate in vitro the specific stages of in vivo human brain development, thus offering an innovative tool by which to model human neurodevelopmental disease. We review here how brain organoids have been used to study neurodevelopmental disease and consider their potential for both technological advancement and therapeutic development.
摘要
类脑器官在体外重现了体内人类大脑发育的特定阶段，因此为模拟人类神经发育性疾病提供了一种创新工具。在这里，我们回顾了类脑器官如何用于研究神经发育性疾病，并考虑了它们在技术进步和治疗开发方面的潜力。
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2
hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids.人胚胎干细胞衍生的丘脑类器官与皮质类器官融合后形成相互投射。
Cell Stem Cell. 2019 Mar 7;24(3):487-497.e7. doi: 10.1016/j.stem.2018.12.015. Epub 2019 Feb 21.
3
Microglia Increase Inflammatory Responses in iPSC-Derived Human BrainSpheres.小胶质细胞增强诱导多能干细胞衍生的人脑球体中的炎症反应。
Front Microbiol. 2018 Dec 4;9:2766. doi: 10.3389/fmicb.2018.02766. eCollection 2018.
4
Reliability of human cortical organoid generation.人类皮质类器官生成的可靠性。
Nat Methods. 2019 Jan;16(1):75-78. doi: 10.1038/s41592-018-0255-0. Epub 2018 Dec 20.
5
Vascularization and Engraftment of Transplanted Human Cerebral Organoids in Mouse Cortex.移植的人类大脑类器官在小鼠大脑皮层中的血管生成和植入。
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6
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8
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