花生四烯酸代谢产物对胸腺细胞增殖的影响。

Effect of arachidonic acid metabolites on thymocyte proliferation.

作者信息

Delebassée S, Gualde N

机构信息

Groupe de Recherche en Immunologie et Biochimie, Limoges, France.

出版信息

Ann Inst Pasteur Immunol. 1988 Jul-Aug;139(4):383-99. doi: 10.1016/0769-2625(88)90065-7.

DOI:10.1016/0769-2625(88)90065-7

PMID:3139013

Abstract

The influences of prostaglandin E2 (PGE2), 15-hydroxyeicosatetraenoic acid (15-HETE) and leukotrienes (LT) on the proliferative response of mature (PNA-) and immature (PNA+) mouse thymocytes was investigated. Both PNA+ and PNA- thymocytes proliferated when cultured with concanavalin A plus interleukin-2. PGE2 in concentrations of 10(-6) to 10(-9) M caused significant inhibition of proliferation of both PNA+ and PNA- thymocytes in these cultures. In contrast, the lipoxygenase products 15-HETE, LTB4, LTC4 and LTD4 caused marked increases in proliferation of PNA+ thymocytes while having no effect on PNA- cells. Therefore, the effect of leukotrienes on thymocyte proliferation depends upon the level of cell maturation and mainly affects immature PNA+ thymocytes.

摘要

研究了前列腺素E2（PGE2）、15-羟基二十碳四烯酸（15-HETE）和白三烯（LT）对成熟（PNA-）和未成熟（PNA+）小鼠胸腺细胞增殖反应的影响。当与伴刀豆球蛋白A加白细胞介素-2一起培养时，PNA+和PNA-胸腺细胞均发生增殖。浓度为10^(-6)至10^(-9) M的PGE2对这些培养物中PNA+和PNA-胸腺细胞的增殖均有显著抑制作用。相反，脂氧合酶产物15-HETE、LTB4、LTC4和LTD4使PNA+胸腺细胞的增殖显著增加，而对PNA-细胞无影响。因此，白三烯对胸腺细胞增殖的影响取决于细胞成熟水平，且主要影响未成熟的PNA+胸腺细胞。