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高增殖胎盘源多能细胞低表达细胞角蛋白 7。

High Proliferative Placenta-Derived Multipotent Cells Express Cytokeratin 7 at Low Level.

机构信息

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, Ukraine.

Institute of Cell Therapy, Kyiv, Ukraine.

出版信息

Biomed Res Int. 2019 Jul 15;2019:2098749. doi: 10.1155/2019/2098749. eCollection 2019.

DOI:10.1155/2019/2098749
PMID:31392209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6662495/
Abstract

The purpose of this study was to investigate the immunophenotypes and gene expression profile of high proliferative placenta-derived multipotent cells (PDMCs) population at different stages of culture. We demonstrated that the colonies resulting from single cells were either positive or negative for CK7, whereas only PDMC clones with weak CK7 expression (CK7-clones) were highly proliferative. Interestingly, vimentin positive (Vim) placental stromal mesenchymal cells did not express CK7 , but double CK7Vim cells detection in tissue explants and explants outgrowth indicated CK7 inducible expression . PCNA presence in CK7Vim cells during placental explants culturing confirmed belonging of these cells to proliferative subpopulation. Transcription factors CDX2 and EOMES were expressed in both CK7-clones and subset of stromal mesenchymal cells of first-trimester placental tissue . Meanwhile, CK7 -clones and stromal mesenchymal cells of full-term placental tissue expressed ERG heterogeneously. , , and mesodermal-related genes expression by CK7-clones additionally confirms their mesenchymal origin. Inherent stem cell-related gene expression ( and ) in CK7-clones might indicate their enrichment for progenitors. Finally, in CK7-clones we observed expression of such trophoblast-associated genes as types I and II, fusogenic , , and . Thus, our results indicate that PDMCs acquired the representative immunophenotype signature under culture conditions.

摘要

本研究旨在探讨不同培养阶段高增殖胎盘多能细胞（PDMCs）群体的免疫表型和基因表达谱。我们证明，由单细胞形成的集落要么 CK7 阳性，要么 CK7 阴性，而只有 CK7 弱表达的 PDMC 克隆（CK7-克隆）才具有高度增殖性。有趣的是，阳性（Vim）胎盘基质间充质细胞不表达 CK7，但组织外植体和外植体生长中 CK7/Vim 双阳性细胞的检测表明 CK7 可诱导表达。在胎盘外植体培养过程中 CK7/Vim 细胞中 PCNA 的存在证实了这些细胞属于增殖亚群。转录因子 CDX2 和 EOMES 在 CK7-克隆和早孕期胎盘组织中一部分基质间充质细胞中表达。同时，CK7-克隆和足月胎盘组织的基质间充质细胞不均匀表达 ERG。CK7-克隆中一些中胚层相关基因的表达进一步证实了它们的间充质来源。CK7-克隆中固有干细胞相关基因（和）的表达可能表明其富含祖细胞。最后，我们在 CK7-克隆中观察到了多种滋养层相关基因的表达，如 I 型和 II 型、融合、和。因此，我们的研究结果表明，PDMCs 在培养条件下获得了代表性的免疫表型特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3628/6662495/5222cbdc7d76/BMRI2019-2098749.008.jpg
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2
Continuous cell supply from Krt7-expressing hematopoietic stem cells during native hematopoiesis revealed by targeted in vivo gene transfer method.通过靶向体内基因转移方法揭示了 Krt7 表达的造血干细胞在天然造血过程中的持续细胞供应。
Sci Rep. 2017 Jan 18;7:40684. doi: 10.1038/srep40684.
3
转移性乳腺导管癌伴神经内分泌分化眶内肿瘤：以眼部症状首发的病例报告及文献复习。
Front Endocrinol (Lausanne). 2021 Feb 22;12:625663. doi: 10.3389/fendo.2021.625663. eCollection 2021.
Stem cell insights into human trophoblast lineage differentiation.
干细胞对人类滋养层谱系分化的研究。
Hum Reprod Update. 2016 Dec;23(1):77-103. doi: 10.1093/humupd/dmw026. Epub 2016 Sep 2.
4
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5
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6
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