Simões Sandra, Lopes Rui, Campos Maria Celeste Dias, Marruz Maria João, da Cruz Maria Eugénia Meirinhos, Corvo Luísa
Faculdade de Farmácia Instituto de Investigação do Medicamento (iMed.ULisboa) Universidade de Lisboa Lisboa Portugal.
Serviço de Anatomia Patológica Hospital Curry Cabral Lisboa Portugal.
Animal Model Exp Med. 2019 Apr 5;2(2):121-126. doi: 10.1002/ame2.12060. eCollection 2019 Jun.
Acute gastric mucosal injury is a common disorder of the gastrointestinal tract and the search for new therapeutics is ongoing. The aim of this study is to update and expand the information related to the most widely used rat models of acute gastric ulcer, the ethanol-induced ulcer and the indomethacin-induced ulcer. These two models are compared in terms of macroscopic and microscopic features. Experimentally, ethanol was given orally in a single dose and indomethacin was subcutaneously injected into male Wistar rats. After ulcerative challenges, the stomachs were removed and visually inspected. Anti-ulcerative drugs were used to validate the models. Histological analysis of the stomachs determined the microscopic score. The methodology used for model evaluation applied to macroscopic and microscopic gastric lesions. With these methods it was possible to induce lesions in the gastric mucosa. Microscopic evaluation permitted assessment of the inflammatory and apoptotic impact in the mucosa not observable by macroscopic evaluation. Groups of animals were treated with two standard drugs: sulcralfate suspension or lansoprazole solution. Both drugs reduced macroscopic and microscopic lesions, particularly the hemorrhagic ones. Both models induced acute gastric mucosal injury and no single evaluation method can address all the aspects of the pathology of gastric lesions. As a complement to macroscopic evaluation, microscopy appears to be a relevant tool to selectively identify specific aspects of the development of mucosal injury, quantify the extent of lesions, and contribute to an appropriate interpretation of results. The score systems established here offer a reliable method for testing antiulcer drugs.
急性胃黏膜损伤是一种常见的胃肠道疾病，目前仍在寻找新的治疗方法。本研究的目的是更新和扩展与最广泛使用的急性胃溃疡大鼠模型（乙醇诱导性溃疡和吲哚美辛诱导性溃疡）相关的信息。对这两种模型的宏观和微观特征进行了比较。实验中，对雄性Wistar大鼠单次口服乙醇，并皮下注射吲哚美辛。在进行溃疡激发后，取出胃并进行肉眼检查。使用抗溃疡药物对模型进行验证。对胃进行组织学分析以确定微观评分。用于模型评估的方法适用于宏观和微观胃损伤。通过这些方法可以在胃黏膜中诱导损伤。微观评估能够评估宏观评估无法观察到的黏膜炎症和凋亡影响。用两种标准药物对动物组进行治疗：硫糖铝混悬液或兰索拉唑溶液。两种药物均能减轻宏观和微观损伤，尤其是出血性损伤。两种模型均诱导了急性胃黏膜损伤，且没有单一的评估方法能够涵盖胃损伤病理学的所有方面。作为宏观评估的补充，显微镜检查似乎是一种相关工具，可用于选择性识别黏膜损伤发展的特定方面、量化损伤程度并有助于对结果进行适当解释。此处建立的评分系统为测试抗溃疡药物提供了一种可靠的方法。
