Liu Xingchen, Zheng Xiang, Liu Yihan, Du Xiaoyan, Chen Zhenwen
School of Basic Medical Science Capital Medical University Beijing China.
Department of Laboratory Animal Capital Medical University Beijing China.
Animal Model Exp Med. 2019 May 14;2(2):127-131. doi: 10.1002/ame2.12068. eCollection 2019 Jun.
The has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring two hormones that show daily oscillations, cortisol and ACTH, in serum using ELISA kits. The levels of the two hormones were highest at 8:00 am and their rhythmic changes were similar to those in humans. In addition, the influence of stress of handling and blood collection on the physiological parameters of the gerbils was examined. After adaptation to handling for 1 week, some serum parameters in the animals changed. Handling and blood collection did not impact significantly on the following parameters: creatine kinase (CK), lactate dehydrogenase (LD), alanine aminotransferase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and albumin (ALB). However, blood glucose (GLU), total protein (TP) and globulin (GLB) significantly increased while creatinine (CRE) and albumin/globulin (A/G) significantly decreased after adaptation. This work further confirms that the is a diurnal animal and also indicates that a suitable adaptation procedure is necessary for getting reliable results when performing experiments using these animals.
该动物已在许多研究领域广泛使用，并且据报道是一种昼行性实验动物。在本研究中，通过使用酶联免疫吸附测定试剂盒测量血清中显示每日振荡的两种激素——皮质醇和促肾上腺皮质激素，来研究这些沙鼠的昼夜节律性。这两种激素的水平在上午8:00最高，并且它们的节律变化与人类相似。此外，还研究了处理和采血应激对沙鼠生理参数的影响。在适应处理1周后，动物的一些血清参数发生了变化。处理和采血对以下参数没有显著影响：肌酸激酶（CK）、乳酸脱氢酶（LD）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、血尿素氮（BUN）和白蛋白（ALB）。然而，适应后血糖（GLU）、总蛋白（TP）和球蛋白（GLB）显著升高，而肌酐（CRE）和白蛋白/球蛋白（A/G）显著降低。这项工作进一步证实该动物是昼行性动物，并且还表明在使用这些动物进行实验时，适当的适应程序对于获得可靠结果是必要的。
