Korabiowska Monika, König Fatima, Schlott Thilo, Slotty Philip, Romeike Bernd, Cordon-Cardo Carlos, Brinck Ulrich
Department of Cytopathology, Georg-August University Göttingen, Robert Koch Str. 40, 37075 Göttingen.
Department of Neuropathology, Georg-August University Göttingen, Robert Koch Str. 40, 37075 Göttingen.
Cancer Genomics Proteomics. 2004 May-Jun;1(3):225-230. Epub 2004 May 1.
Altered expression and prognostic significance of DNA double-strand repair genes Ku70 and Ku80 has been shown by our research group for malignant melanomas. High genomic instability known for melanoma brain metastases stimulated us to analyze Ku70 and Ku80 expression in melanoma brain metastases.
Quantitative evaluation of mRNA Ku70 and Ku80 expression was performed in 13 melanoma brain metastases. Immunohistochemistry and in situ hybridization for Ku70 and Ku80 were applied to 34 metastatic tumours.
Quantitative analysis of Ku70 mRNA expression demonstrated values between 0.01 and 0.33. Ku80 mRNA expression ranged between 0.001 and 0.54. Immunohistochemistry demonstrated Ku70 and Ku80 expression in 34 and in 25 metastatic tumours, respectively. In situ hybridization detected Ku70 expression in 19/34 and Ku80 expression in 13/34 metastatic tumours. Correlation between Ku70 and Ku80 expression in melanoma brain metastases was lost.
Ku70 and especially Ku80 expression is altered in melanoma brain metastases and corresponds with the high genomic instability of these lesions.
我们的研究小组已表明，DNA双链修复基因Ku70和Ku80的表达改变及其在恶性黑色素瘤中的预后意义。黑色素瘤脑转移以高基因组不稳定性著称，这促使我们分析Ku70和Ku80在黑色素瘤脑转移中的表达。
对13例黑色素瘤脑转移进行Ku70和Ku80 mRNA表达的定量评估。对34例转移性肿瘤进行Ku70和Ku80的免疫组织化学及原位杂交检测。
Ku70 mRNA表达的定量分析显示其值在0.01至0.33之间。Ku80 mRNA表达范围在0.001至0.54之间。免疫组织化学显示，分别有34例和25例转移性肿瘤表达Ku70和Ku80。原位杂交在19/34例转移性肿瘤中检测到Ku70表达，在13/34例中检测到Ku80表达。黑色素瘤脑转移中Ku70和Ku80表达之间的相关性消失。
Ku70尤其是Ku80的表达在黑色素瘤脑转移中发生改变，这与这些病变的高基因组不稳定性相符。
